Method Library

This resource aims to make methods more accessible to researchers, and help identify the most relevant workflows for your current application. We would appreciate your participation in keeping this database up to date, so please report any errors such as broken links, missing information, or publications that are not included to info@resynbio.com.

Resource TitleYearResourceApplicationProductSample SourceStrategyExtractionClean-upDigestionQuantification
Preparation and HILIC SPE clean-up of urinary proteins for MS analysis2023MethodDigest preparationHILICBiofluidsManualDetergentHydrophilic affinity LysC, Trp, On-beadLabel-free
Tissue-wide Effects Overrule Cell-intrinsic Gene Function in Cortical Projection Neuron Migration2022PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualKitKitTrpIsobaric-tagging
ULK4 and Fused/STK36 interact to mediate assembly of a motile flagellum 2022PublicationBioIDStreptavidinMosquito cellsManualDetergentAffinity captureN/ALabel-free
Assessment of Streptavidin Bead Binding Capacity to Improve Quality of Streptavidin-based Enrichment Studies2021PublicationAffinity captureStreptavidinN/AN/AN/AN/AN/ALabel-free
Liver-fibrosis-activated transcriptional networks govern hepatocyte reprogramming and intra-hepatic communication2021PublicationDigest preparationHILICMammalian tissueManualChaotropePrecipitation on-beadLysC, Trp, On-beadLabel-free
Bromodomain factor 5 is an essential regulator of transcription in Leishmania2022PublicationBioID, Phosphopeptide enrichmentStreptavidin, Ti-IMACLeishmania protozoa ManualDetergentAffinity captureTrp, On-beadLabel-free
Dissecting the Functional Selectivity of TGF? Signaling Pathway Components Using Genome A2021PublicationDigest preparation, Phosphopeptide enrichmentAmine, Ti-IMACMammalian tissueAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadIsobaric-tagging
Loquacious Modulates Flaviviral RNA Replication in Mosquito Cells2021PublicationAffinity captureStreptavidinInsect cellsManualDetergentAffinity captureN/ALabel-free
Dysregulated healing responses in diabetic wounds occur in the early stages postinjury2021PublicationDigest preparationHILICMammalian tissueManualOrganicHydrophilic affinityTrpIsobaric-tagging
The cGMP-Dependent Protein Kinase 2 Contributes to Cone Photoreceptor Degeneration in the Cnga3-Deficient Mouse Model of Achromatopsia2021PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualN/APrecipitation in-solutionTrpLabel-free
Taylor-made production of pyrimidine nucleoside-5′-monophosphate analogues by highly stabilized mutant uracil phosphoribosyltransferase from Toxoplasma gondii2021PublicationImmobilizationAmine, Ni-NTAEnzymeN/AN/AN/AN/AN/A
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases2021PublicationImmobilizationAmineEnzymeN/AN/AN/AN/AN/A
Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution2021Publication Digest preparation, Phosphopeptide enrichmentAmine, Ti-IMACMammalian tissueAutomatedNative, DetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci2021PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentPrecipitation in-solutionTrpMetabolic labelling
Identifying cytokine signaling signatures in primary human Th-1 cells by phospho-proteomics analysis2021PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentPrecipitation in-solutionTrpMetabolic labelling
Molecular roadmap of the healthy stress response in the mouse hippocampus2021PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualDetergentFilter-basedTrpLabel-free
HNF4A is required to specify glucocorticoid action in the liver2021PublicationChIPProtein GMammalian tissueManualN/AN/AN/AN/A
Rapid auxin-mediated phosphorylation of Myosin regulates trafficking and polarity in Arabidopsis2021PublicationPhosphopeptide enrichmentTiO2, Ti-IMACPlant ManualDetergentHybridTrpLabel-free
Venom Proteomic Analysis of Two Medically Important Nigerian Viper (Echis Ocellatus and Bitis Arietans) Snake Species2021PublicationDigest preparationHILICSnake venomManualN/AHybridTrpLabel-free
Monocyte Released HERV-K dUTPase Engages TLR4 and MCAM Causing Endothelial Mesenchymal Transition2021PublicationPeptide clean-upHILICMammalian cellsManualNativeHydrophilic affinityLysC, Trp, On-beadLabel-free
Proteomics of resistance to Notch1 inhibition in acute lymphoblastic leukemia reveals targetable kinase signatures2021PublicationDigest preparation, Phosphopeptide enrichmentAmine, Ti-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadIsobaric-tagging, Label-free
KF Method: Automated Phosphopeptide Enrichment using MagReSyn® IMAC 2021MethodPhosphopeptide enrichmentTi-IMAC, Zr-IMACN/AAutomatedN/AN/AN/ALabel-free
High-throughput phosphoproteomics profiling of non-small cell lung cancer tissues2021PosterPhosphopeptide enrichmentTi-IMACMammalian tissueAutomatedN/AIn-solutionTrpLabel-free
The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted antiviral signaling and host translation2021PublicationBioIDStreptavidinMammalian cellsAutomatedDetergentAffinity captureLysC, TrpLabel-free
Product Guide MagReSyn® Trypsin2021MethodDigest preparationTrypsinN/AManualN/AIn-solutionTrpN/A
Product Guide MagReSyn® Chymotrypsin2021MethodDigest preparationChymotrypsinRecombinant protein ManualN/AIn-solutionChymoTrpN/A
Competitive binding of STATs to receptor phospho-Tyr motifs accounts for altered cytokine responses2021PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentPrecipitation in-solutionTrpMetabolic labelling
Drug-bound and -free outward-facing structures of a multidrug ABC exporter point to a swing mechanism2021PublicationHDXZr-IMACRecombinant protein N/AN/AN/APepsinHDX
Antagonistic cell surface and intracellular auxin signalling regulate plasma membrane H+ fluxes for root growth2021PublicationPhosphopeptide enrichmentTi-IMACPlant ManualDetergentFilter-basedTrpLabel-free
The membrane-localized protein kinase MAP4K4/TOT3 regulates thermomorphogenesis2021PublicationPhosphopeptide enrichmentTi-IMACPlant ManualNativePrecipitation in-solutionLysC, TrpLabel-free
The cGMP-Dependent Protein Kinase 2 Contributes to Cone Photoreceptor Degeneration in the Cnga3-Deficient Mouse Model of Achromatopsia2021PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualN/APrecipitation in-solutionTrpLabel-free
Unraveling the MAX2 Protein Network in Arabidopsis thaliana: Identification of the Protein Phosphatase PAPP5 as a Novel MAX2 Interactor2021PublicationPhosphopeptide enrichmentTi-IMACPlant ManualNativeAffinity captureLysC, TrpLabel-free
An AKT2-specific nanobody that targets the hydrophobic motif induces cell cycle arrest, autophagy and loss of focal adhesions in MDA-MB-231 cells2021PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualChaotropeIn-solutionLysC, TrpLabel-free
Evaluation of Protein Purification Techniques and Effects of Storage Duration on LC MS MS Analysis of Archived FFPE Human CRC Tissues2021PublicationDigest preparationHILICFFPEAutomatedDetergentHydrophilic affinity Trp, On-beadLabel-free
Functional studies of CpSRP54 in diatoms show that themechanism of thylakoid protein insertion differs from that inplants and green algae2021PublicationDigest preparationHILICMammalian cellsAutomatedDetergentHydrophilic affinity Trp, On-beadLabel-free
Proteomic analysis of three medically important Nigerian Naja (Naja haje, Naja katiensis and Naja nigricollis) snake venoms2021PublicationDigest preparationHILICSnake venomManualN/AHydrophilic affinity Trp, On-beadLabel-free
Plasmodium falciparum Calcium Dependent Protein Kinase 4 is critical for male gametogenesis and transmission to the mosquito vector2021PublicationPhosphopeptide enrichmentTi-IMAC, Zr-IMACPlasmodiumAutomatedDetergentPrecipitation in-solutionTrpLabel-free
Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion2021PublicationDigest preparation, Phosphopeptide enrichmentHILIC, Ti-IMACMammalian cells, OtherManualDetergentPrecipitation on-filter, Affinity captureTrpIsobaric-tagging, Label-free
DYRK3-Controlled Phase Separation Organizes the Early Secretory Pathway2020PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsAutomatedN/AOtherTrpLabel-free
Phosphorylation controls RNA binding and transcription by the influenza virus polymerase2020PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualChaotropePrecipitation in-solutionTrpLabel-free
A Compact Quadrupole-Orbitrap Mass Spectrometer with FAIMS Interface Improves Proteome Coverage in Short LC Gradients2020PublicationDigest preparation, Phosphopeptide enrichmentAmine, Ti-IMACMammalian cells, Mammalian tissueAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Chemical Genetics of AGC-kinases Reveals Shared Targets of Ypk1, Protein Kinase A and Sch92020PublicationPhosphopeptide enrichmentTi-IMAC, TiO2, ZrO2 Yeast cellsManualN/APrecipitation in-solutionTrpLabel-free
Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries2020PublicationPhosphopeptide enrichmentTi-IMACYeast cellsManualDetergentPrecipitation in-solutionLysC, TrpLabel-free
Functional studies of CpSRP54 in diatoms show that the mechanism of thylakoid protein insertion differs from that in plants and green algae2020PublicationDigest preparationHILICAlgaeAutomatedDetergentHydrophilic affinityTrp, On-beadLabel-free
SWATH-MS based proteomic profiling of pancreatic ductal adenocarcinoma tumours reveals the interplay between the extracellular matrix and related intracellular pathways2020PublicationDigest preparationHILICMammalian tissueAutomatedDetergentHydrophilic affinityTrp, On-beadLabel-free
CDK8 fine-tunes IL-6 transcriptional activities by limiting STAT3 resident time at the gene loci2020PublicationPhosphopeptide enrichmentTi-IMACPlant ManualNativePrecipitation in-solutionLysC, TrpLabel-free
The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis2020PublicationPhosphopeptide enrichmentTi-IMACPlant ManualNativePrecipitation in-solutionLysC, TrpLabel-free
Alterations in the CO2 availability induce alterations in the phosphoproteome of the cyanobacterium Synechocystis sp. PCC 68032020PublicationPhosphopeptide enrichmentTi-IMAC, TiO2Bacterial cellsManualDetergentPrecipitation in-solutionLysC, TrpLabel-free
CYCA3;4 Is a Post-Prophase Target of the APC/CCCS52A2 E3-Ligase Controlling Formative Cell Divisions in Arabidopsis2020PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentN/ATrpMetabolic labelling
HIPK4 is essential for murine spermiogenesis2020PublicationPhosphopeptide enrichmentTi-IMAC, TiO2Mammalian cellsManualDetergentPrecipitation in-solutionLysC, TrpIsobaric-tagging
Fluorescence fluctuation analysis reveals PpV dependent Cdc25 protein dynamics in living embryos2020PublicationPhosphopeptide enrichmentTi-IMACDrosophilaManualNativeGel-basedTrp (in-gel)Label-free
Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B2020PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsAutomatedDetergentPrecipitation in-solutionLysC, TrpIsobaric-tagging
Cannabinoid exposure in rat adolescence reprograms the initial behavioral, molecular, and epigenetic response to cocaine2020PublicationPhosphopeptide enrichmentTi-IMAC, Zr-IMACMammalian tissueManualChaotropePrecipitation in-solutionLysC, TrpIsobaric-tagging
The Diabetes Gene JAZF1 Is Essential for the Homeostatic Control of Ribosome Biogenesis and Function in Metabolic Stress2020PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentFilter-basedN/ALabel-free
Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc2020PublicationPhosphopeptide enrichmentTi-IMAC, TiO2, ZrO2Yeast cellsManualChaotropeIn-solutionLysC, TrpMetabolic labelling
An Unbiased Approach to Mapping the Signaling Network of the Pseudorabies Virus US3 Protein2020PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualChaotropeIn-solutionLysC, TrpLabel-free
Zirconium(IV)-IMAC Revisited: Improved Performance and Phosphoproteome Coverage by Magnetic Microparticles for Phosphopeptide Affinity Enrichment2020PublicationPhosphopeptide enrichmentTi-IMAC, Zr-IMAC, TiO2 Mammalian cellsBothDetergentPrecipitation in-solutionTrpLabel-free
Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in translation2020PublicationPhosphopeptide enrichmentTiO2Plant ManualNativeIn-solutionTrpIsobaric-tagging
Magnetic micro-macro biocatalysts applied to industrial bioprocesses2020PublicationImmobilizationNi-NTARecombinant protein ManualN/AN/AN/AN/A
Magnetic micro-macro biocatalysts applied to industrial bioprocesses2020PublicationImmobilizationAmineRecombinant protein ManualN/AN/AN/AN/A
Novel kinase platform for the validation of the anti-tubercular activities of Pelargonium sidoides (Geraniaceae)2020PublicationProtein PurificationNi-NTABacterial cellsManualNativeN/AN/AN/A
Product Guide MagReSyn® TiO22022MethodPhosphopeptide enrichmentTiO2N/AManualN/AN/AN/ALabel-free
Evaluation of Novel Supports for Selective and Efficient Enrichment of Phosphorylated Peptides2020MethodPhosphopeptide enrichmentTiO2, ZrO2N/AAutomatedN/AN/AN/ALabel-free
Product Guide MagReSyn® ZrO22022MethodPhosphopeptide enrichmentZrO2N/AManualN/AN/AN/ALabel-free
Product Guide MagReSyn® Ti-IMAC HP2022MethodPhosphopeptide enrichmentTi-IMACN/AManualN/AN/AN/ALabel-free
Product Guide MagReSyn® Ti-IMAC2022MethodPhosphopeptide enrichmentTi-IMACN/AManualN/AN/AN/ALabel-free
A Data Independent Acquisition Workflow Enables the Profiling of Thousands of Human Cancer Tissues for Precision Oncology2020PosterPhosphopeptide enrichmentTi-IMACMammalian tissueAutomatedUnknownIn-solutionTrpLabel-free
Fast and reproducible phosphoproteomics using MagReSyn® Amine and Ti-IMAC HP magnetic beads and the Evosep One2020Application noteDigest preparation, Phosphopeptide enrichmentAmine, Ti-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
High Throughput Proteome and Phosphoproteome Sample Processing Couple to Fast Gradient DIA2020PosterDigest preparation, Phosphopeptide enrichmentAmine, Ti-IMACMammalian tissueAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Product Guide MagReSyn® Zr-IMAC HP2022MethodPhosphopeptide enrichmentZr-IMACN/AManualN/AN/AN/ALabel-free
Product Guide MagReSyn® Zr-IMAC2022MethodPhosphopeptide enrichmentZr-IMACN/AManualN/AN/AN/ALabel-free
Zirconium(IV) IMAC-based enrichment for mass spectrometry driven phosphoproteomics2020PosterPhosphopeptide enrichmentTi-IMAC, Zr-IMACMammalian cellsAutomatedN/AN/AN/ALabel-free
KF Method: Automated clean-up and on-bead digestion with MagReSyn® HILIC2020MethodDigest preparationHILICN/AAutomatedDetergentHydrophilic affinity Trp, On-beadLabel-free
Sustainable synthesis of uridine-5?-monophosphate analogues by immobilized uracil phosphoribosyltransferase from Thermus thermophilus2020PublicationImmobilizationAmineRecombinant protein ManualN/AN/AN/AN/A
Identification of MAMP-Responsive Plasma Membrane-Associated Proteins in Arabidopsis thaliana Following Challenge with Different LPS Chemotypes from Xanthomonas campestris2020PublicationAffinity captureStreptavidinBacterial cellsManualN/AN/AN/AN/A
Biochemical Pulldown of mRNAs and Long Noncoding RNAs from Cellular Lysates Coupled with Mass Spectrometry to Identify Protein Binding Partners2020PublicationAffinity captureStreptavidinMammalian cellsManualDetergentGel-basedTrpLabel-free
Multiplexed Proximity Biotinylation Coupled to Mass Spectrometry for Defining Integrin Adhesion Complexes2020PublicationBioIDStreptavidinMammalian cellsManualDetergentGel-basedTrpLabel-free
Topological features of integrin adhesion complexes revealed by multiplexed proximity biotinylation2020PublicationBioIDStreptavidinMammalian cellsManualDetergentGel-basedTrpLabel-free
BioID based proteomic analysis of the Bid interactome identifies novel proteins involved in cell cycle dependent apoptotic priming2020PublicationBioIDStreptavidinMammalian cellsManualDetergentGel-basedTrpLabel-free
Plant-based production of highly potent anti-HIV antibodies with engineered posttranslational modifications2020PublicationAffinity captureProtein APlant ManualNativeN/AN/AN/A
Hypoxia regulates GR function through multiple mechanisms involving microRNAs 103 and 1072020PublicationChIPProtein GMammalian cellsManualN/AN/AN/AqPCR
Insights from the First Phosphopeptide Challenge of the MS Resource Pillar of the HUPO Human Proteome Project2020PublicationPhosphopeptide enrichmentTi-IMACHUPO SyntheticBothN/AN/AN/AN/A
Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis2019PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualChaotropePrecipitation in-solutionTrp double digestIsobaric-tagging
Proteasome-mediated remodeling of the proteome and phosphoproteome during kiwifruit pollen germination2019PublicationPhosphopeptide enrichmentTi-IMACPlant ManualN/AHybridN/ALabel-free
Conduit integrity is compromised during acute lymph node expansion2019PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsAutomatedChaotropeFilter-basedN/AIsobaric-tagging
Probability-based detection of phosphoproteomic uncertainty reveals rare signaling events driven by oncogenic kinase gene fusion2019PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentPrecipitation in-solutionTrpLabel-free
CDK8 Regulates Insulin Secretion and Mediates Postnatal and Stress-Induced Expression of Neuropeptides in Pancreatic ? Cells2019PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentFilter-basedN/ALabel-free
Protein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation2019PublicationDigest preparation, Phosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
R2-P2 rapid-robotic phosphoproteomics enables multidimensional cell signaling studies2019PublicationPhosphopeptide enrichmentTi-IMAC, Zr-IMAC, TiO2Yeast cellsAutomatedChaotropePrecipitation on-beadLysC, Trp, On-beadLabel-free
Development of fully automated pipeline for phosphoproteome profiling optimisation of phosphopeptide enrichment2019PosterPhosphopeptide enrichmentTi-IMAC, Zr-IMAC, TiO2, ZrO2, Amine, HILIC, SAXRecombinant protein, Mammalian cellsAutomatedDetergent, ChaotropeIn-solution, Precipitation on-bead, Hydrophilic affinity, Ion exchangeTrpIsotope-labelled peptides, Label-free
Development of a fully automated high throughput magnetic workflow for phosphoproteome profiling2019PosterPhosphopeptide enrichmentTi-IMAC, Zr-IMAC, TiO2Mammalian cellsAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
Development of fully automated pipeline for phosphoproteome profiling2019PosterDigest preparation, Phosphopeptide enrichment Ti-IMAC, SAX, HILICMammalian cellsAutomatedDetergent, ChaotropePrecipitation on-bead, Hydrophilic affinity, Ion exchange TrpLabel-free
PEPCLU MagReSyn HILIC Clean-Up of Peptides Post Protein Digestion2019MethodPeptide clean-upHILICN/AManualDetergentHydrophilic affinity N/ALabel-free
RAPOBD HILIC Clean-Up of Reduced & Alkylated Proteins with on Bead Digestion2019MethodDigest preparationHILICMammalian cellsManualDetergentHydrophilic affinity Trp, On-beadLabel-free
POBRAD HILIC Clean-Up of proteins with on-bead reduction, alkylation and digestion2019MethodDigest preparationHILICMammalian cellsManualDetergentHydrophilic affinity Trp, On-beadLabel-free
Improved reproducibility of automated sample preparation using HILIC magnetic beads automated by Hamilton Robotics2019Application noteDigest preparationHILICN/AAutomatedDetergentHydrophilic affinity Trp, On-beadLabel-free
Development of a fully automated magnetic workflow for phosphoproteome profiling2019PosterDigest preparation, Phosphopeptide enrichmentTi-IMAC, Zr-IMAC, TiO2, ZrO2, SAX, HILICMammalian cellsAutomatedDetergent, ChaotropeHydrophilic affinity, Ion exchangeTrp, On-beadLabel-free
Product Guide MagResyn® Amine - activation with epoxide for protein immobilization2019MethodImmobilizationAmineN/AManualN/AN/AN/AN/A
Product Guide MagReSyn® Streptavidin2021MethodAffinity captureStreptavidinN/AManualN/AN/AN/AN/A
Product Guide MagReSyn® Streptavidin MAX2021MethodAffinity captureStreptavidinN/AManualN/AN/AN/AN/A
Product Guide MagReSyn® Streptavidin for MS2023MethodAffinity captureStreptavidinN/AManualN/AN/AN/AN/A
Product Guide MagReSyn® Protein A2021MethodAffinity captureProtein AN/AManualN/AN/AN/AN/A
Product Guide MagReSyn® Protein A MAX2021MethodAffinity captureProtein AN/AManualN/AN/AN/AN/A
Product Guide MagReSyn® Protein G2021MethodAffinity captureProtein GN/AManualN/AN/AN/AN/A
p38α MAPK proximity assay reveals a regulatory mechanism of alternative splicing in cardiomyocytes2019PublicationAffinity captureProtein GMammalian tissueManualDetergentGel-basedN/AN/A
An improved method for quantitative ChIP studies of nuclear receptor function2019PublicationChIPProtein GMammalian tissueManualN/AIn-solutionN/AqPCR
The Auxin-Regulated CrRLK1L Kinase ERULUS Controls Cell Wall Composition during Root Hair Tip Growth2018PublicationPhosphopeptide enrichmentTi-IMACPlant ManualNativePrecipitation in-solutionLysC, TrpLabel-free
Ultra-High Pressure (>30,000 psi) Packing of Capillary Columns Enhancing Depth of Shotgun Proteomic Analyses2018PublicationPhosphopeptide enrichmentTi-IMACMammalian cells, Mammalian tissueManualChaotropePrecipitation in-solutionLysC, TrpIsobaric-tagging
Magnetic HILIC: An enabling & versatile tool for robust automated MS sample preparation workflows2018PosterDigest preparation, Peptide clean-upHILICMammalian cellsAutomatedDetergentHydrophilic affinity Trp, On-beadLabel-free
Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives2018PublicationImmobilizationAmineRecombinant protein ManualN/AN/AN/AN/A
Enzymatic Synthesis of Therapeutic Nucleosides using a Highly Versatile Purine Nucleoside 2’-DeoxyribosylTransferase from Trypanosoma brucei2018PublicationImmobilizationAmineRecombinant protein ManualN/AN/AN/AN/A
One-Pot, One-Step Production of Dietary Nucleotides by Magnetic Biocatalysts2018PublicationImmobilizationAmineRecombinant protein ManualN/AN/AN/AN/A
Immune genes are primed for robust transcription by proximal long noncoding RNAs located in nuclear compartments2018PublicationAffinity captureStreptavidin, Protein A, Protein GMammalian cellsManualDetergentGel-basedN/AqPCR
REVERBa couples the circadian clock to hepatic glucocorticoid action2018PublicationChIP, Affinity captureProtein A, Protein GMammalian cellsManualN/AN/AN/AqPCR
LRP1 is required for novobiocin-mediated fibronectin turnover2018PublicationAffinity captureProtein AMammalian cellsManualDetergentGel-basedTrpN/A
Circadian clock component REV-ERB? controls homeostatic regulation of pulmonary inflammation2018PublicationAffinity captureProtein AMammalian cellsManualDetergentGel-basedTrpN/A
Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation2017PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsAutomatedDetergentPrecipitation in-solutionTrp, AspN Isobaric-tagging
Phosphoproteomics with Activated Ion Electron Transfer Dissociation2017PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualChaotropePrecipitation in-solutionTrpLabel-free
Proteome Profiling of Wheat Shoots from Different Cultivars2017PublicationPhosphopeptide enrichmentTi-IMACPlant ManualNativePrecipitation in-solutionLysC, TrpLabel-free
GSK3α and GSK3β Phosphorylate Arc and Regulate its Degradation2017PublicationPhosphopeptide enrichmentTi-IMACRecombinant protein ManualDetergentOtherTrp, PepsinLabel-free
Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid Chromatography–Tandem Mass Spectrometry2017PublicationEnrichment of ADP-ribosylation Ti-IMACRecombinant protein ManualChaotropeFilter-basedTrpLabel-free
Cell-Specific Labeling for Analyzing Bidirectional Signaling by Mass Spectrometry2017PublicationPhosphopeptide enrichmentTi-IMAC, TiO2Mammalian cellsManualChaotropeFilter-basedTrpMetabolic labelling
Comparative genetic, proteomic and phosphoproteomic analysis of C. elegans embryos with a focus on ham-1/STOX and pig-1/MELK in dopaminergic neuron development2017PublicationPhosphopeptide enrichmentTi-IMACC. elegansAutomatedChaotropeIn-solutionLysC, TrpLabel-free
HIV-1 Activates T Cell Signaling Independently of Antigen to Drive Viral Spread2017PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentFilter-basedTrp double digestMetabolic labelling
Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS2017PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentPrecipitation on-beadLysC, Trp, On-beadIsobaric-tagging
Product Guide MagReSyn® HILIC2019MethodDigest preparationHILICN/AManualDetergentHydrophilic affinity Trp, On-beadLabel-free
Reactivation of MagReSyn® Ti-IMAC2017MethodPhosphopeptide enrichmentTi-IMACN/AN/AN/AN/AN/AN/A
Universal Unbiased pre-MS Clean-Up Using Magnetic HILIC Microparticles for SPE2017PosterDigest preparationHILICMammalian cellsAutomatedDetergentHydrophilic affinity Trp, On-beadLabel-free
Dealing with the Dirty Dozen: Universal Unbiased preMS Clean-Up Using Magnetic HILIC SPE2017PosterDigest preparationHILICMammalian cellsAutomatedDetergentHydrophilic affinity Trp, On-beadLabel-free
Product Guide MagReSyn® Amine2017MethodImmobilizationAmineN/AManualN/AN/AN/AN/A
Structural and functional characterization of thermostable biocatalysts for the synthesis of 6-aminopurine nucleoside-5?-monophospate analogues2017PublicationImmobilizationAmineRecombinant protein ManualN/AN/AN/AN/A
Product Guide MagReSyn® Carboxyl2017MethodImmobilizationCarboxylRecombinant protein ManualN/AN/AN/AN/A
Identification of lipopolysaccharide-interacting plasma membrane-type proteins in Arabidopsis thaliana2017PublicationAffinity captureStreptavidinPlant ManualNativeGel-basedTrpN/A
Up-to-Date Workflow for Plant (Phospho)proteomics Identifies Differential Drought-Responsive Phosphorylation Events in Maize Leaves2016PublicationPhosphopeptide enrichmentTi-IMACPlant ManualNativePrecipitation in-solutionLysC, TrpLabel-free
Oncogenic KRAS Regulates Tumor Cell Signaling via Stromal Reciprocation2016PublicationPhosphopeptide enrichmentTi-IMAC, TiO2Mammalian cellsAutomatedChaotropeFilter-basedLysC, TrpIsobaric-tagging
The co-immobilization of P450-type nitric oxide reductase and glucose dehydrogenase for the continuous reduction of nitric oxide via cofactor recycling2016PublicationImmobilizationAmineRecombinant protein ManualN/AN/AN/AN/A
The co-immobilization of P450-type nitric oxide reductase and glucose dehydrogenase for the continuous reduction of nitric oxide via cofactor recycling2016PublicationImmobilizationCarboxylRecombinant protein ManualN/AN/AN/AN/A
An Unbiased Mass Spectrometry Approach Identifies Glypican-3 as an Interactor of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR) in Hepatocellular Carcinoma Cells2016PublicationAffinity captureProtein GMammalian cellsManualNativeGel-basedTrpN/A
Phosphoproteomic analysis of interacting tumor and endothelial cells identifies regulatory mechanisms of transendothelial migration2016PublicationAffinity captureProtein AMammalian cellsManualDetergentN/AN/AN/A
Comprehensive protein sequence coverage using MagReSyn® Trypsin & MagReSyn® Chymotrypsin2016Application noteDigest preparationTrypsinRecombinant protein AutomatedChaotropeIn-solutionTrpLabel-free
Comprehensive protein sequence coverage using MagReSyn® Trypsin & MagReSyn® Chymotrypsin2016Application noteDigest preparationChymotrypsinRecombinant protein AutomatedChaotropeIn-solutionChymoTrpLabel-free
Improved stability of MagReSyn® Trypsin in the presence of denaturants: SDS and Urea 2016Application noteDigest preparationTrypsinRecombinant protein ManualChaotropeIn-solutionTrpLabel-free
Efficient purification of polyhistidinetagged proteins under denaturing conditions using MagReSyn® NTA Inclusion bodies & hidden tags2015Application noteProtein PurificationNi-NTABacterial cellsManualChaotropeN/AN/AN/A
MagReSyn NTA Increased purity of histidine-tagged proteins through enhanced binding affinity2015Application noteProtein PurificationNi-NTABacterial cellsManualNativeN/AN/AN/A
Product Guide MagReSyn® NTA Screening Kit2015MethodProtein PurificationNTA-KitBacterial cellsManualNativeN/AN/AN/A
Product Guide MagReSyn® NTA2015MethodProtein PurificationNi-NTABacterial cellsManualNativeN/AN/AN/A
A colorimetric-based amplification system for proteinases including MMP2 and ADAM82015PublicationImmobilizationNi-NTAMammalian cellsManualNativeN/AN/AN/A
Product Guide MagReSyn® SAX2015MethodProtein PurificationSAXN/AManualNativeN/AN/AN/A
Immobilization of thermostable nucleoside phosphorylases on MagReSyn® epoxide microspheres and their application for the synthesis of 2,6-dihalogenated purine nucleosides2015PublicationImmobilizationAmineRecombinant protein ManualN/AN/AN/AN/A
Synthesis of 2,6-Dihalogenated Purine Nucleosides by Thermostable Nucleoside Phosphorylases2015PublicationImmobilizationAmineRecombinant protein ManualN/AN/AN/AN/A
Ultrasensitive proteome analysis using paramagnetic bead technology2015PublicationDigest preparationCarboxylDrosophilaManualDetergentPrecipitation on-beadTrp, On-beadLabel-free
Reproducible Automated Phosphopeptide Enrichment Using Magnetic TiO2 and Ti-IMAC2014PublicationPhosphopeptide enrichmentTi-IMAC, TiO2Mammalian cellsAutomatedN/AFilter-basedLysC, TrpMetabolic labelling
Cell-specific Labeling Enzymes for Analysis of Cell–Cell Communication in Continuous Co-culture2014PublicationPhosphopeptide enrichmentTi-IMAC, TiO2Mammalian cellsManualChaotropeFilter-basedLysC, TrpMetabolic labelling
Spectrophotometric activity microassay for pure and recombinant cytochrome P450-type nitric oxide reductase2014PublicationProtein PurificationNi-NTABacterial cellsManualDetergentN/AN/AN/A
Robotic Platform for Parallelized Cultivation and Monitoring of Microbial Growth Parameters in Microwell Plates2014PublicationProtein PurificationNi-NTABacterial cellsAutomatedNativeN/AN/AN/A
Effective immunotherapy of rat glioblastoma with prolonged intratumoral delivery of exogenous heat shock protein Hsp702014PublicationAffinity captureStreptavidinMammalian tissueManualNativeIn-solutionN/AN/A
PASTA kinase-dependent control of peptidoglycan synthesis via ReoM is required for cell wall 2021PublicationPhosphopeptide enrichmentTiO2, Zr-IMACBacterial cellsManualDetergentPrecipitation in-solutionTrpLabel-free
Multi-omic analysis reveals the biochemical changes underpinning the varied phenotypes of the Arabidopsis long-period mutant rve 4 6 82021PublicationPhosphopeptide enrichmentTi-IMACPlantAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
A heparan sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells2021PublicationAffinity captureStreptavidinMammalian cellsManualDetergentAffinity captureLysC, TrpLabel-free
A complete and automated sample preparation strategy for high throughput and standardized proteomics2021PosterDigest preparationHydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
A flexible workflow for building spectral libraries from narrow window data independent 2021PublicationDigest preparationAmineMammalian cellsAutomatedChaotropePrecipitation on-beadTrp, On-beadLabel-free
A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant Xerophyta 2021PublicationDigest preparationHILICPlantAutomatedDetergentHydrophilic affinityTrp, On-beadLabel-free
Early mannitol-triggered changes in the Arabidopsis leaf (phospho)proteome reveal growth 2018PublicationPhosphopeptide enrichmentTi-IMACPlantManualNativePrecipitation in-solutionLysC, TrpLabel-free
Plant Phosphoproteomics: Known Knowns, Known Unknowns, and Unknown Unknowns of an Emerging Systems Science Frontier2021PublicationPhosphopeptide enrichmentTi-IMACPlantN/AN/AN/AN/AN/A
Laboratory Protocol: Protein Aggregation Capture (PAC) 2021MethodDigest preparationHydroxylN/AManualDetergentPrecipitation on-beadLysC, Trp, On-beadN/A
Rapid factor depletion highlights intricacies of nucleoplasmic RNA degradation2022PublicationDigest preparationHydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, TrpIsobaric-tagging
Unraveling virus-induced cellular signaling cascades by label-free quantitative phosphoproteomics2022PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManual, AutomatedDetergentFilter-basedTrpLabel-free
Phosphoproteomic profiling of influenza virus entry reveals infection-triggered filopodia 2022PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsAutomated ChaotropeFilter-basedTrpLabel-free
Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy2022PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualSubcellular fractionationPrecipitationLysC, TrpIsobaric-tagging
STK25 inhibits PKA signaling by phosphorylating PRKAR1A2022PublicationPhosphopeptide enrichmentTi-IMAC, Zr-IMACMammalian cellsManualChaotrope, DetergentPrecipitationLysC, TrpIsobaric-tagging
Comparison of characteristics and tumor targeting properties of extracellular vesicles derived from primary NK cells or NK-cell lines stimulated with IL-15 or IL-12/15/182022PublicationDigest preparationAmineExtracellular vesiclesManualDetergentPrecipitation on-beadTrpLabel-free
Multi-omic analysis of the Arabidopsis clock activator mutant rve 4 6 8 reveals connections to carbohydrate metabolism and proteasome regulation2022PublicationPhosphopeptide enrichmentTi-IMACPlantAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
Systems level analysis of time and stimuli specific signaling through PKA2022PublicationPhosphopeptide enrichmentTi-IMACYeast cellsManualChaotrope, DetergentIn-solutionLysC, TrpLabel-free
Covalent coupling of Antibody to MagReSyn Carboxyl ferromagnetic microparticles using Anteobind™2022MethodImmobilizationCarboxylN/AManualN/AN/AN/AN/A
Phosphorylation of SHP2 at Tyr62 enables acquired resistance to SHP2 allosteric inhibitors in FLT3-ITD-driven AML2022PublicationDigest preparation, Phosphopeptide enrichmentTi-IMAC, AmineMammalian tissueAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Multiomic profiling of the acute stress response in the mouse hippocampus2022PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueAutomatedDetergentFilter-basedTrp
The EGFR/ErbB inhibitor neratinib modifies the neutrophil phosphoproteome and promotes apoptosis and clearance by airway macrophages2022PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentPrecipitation on-beadTrp, On-beadLabel-free
A Non-Hazardous Deparaffinization Protocol Enables Quantitative Proteomics of Core Needle Biopsy-Sized Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens2022PublicationDigest preparationAmineFFPEManualDetergentPrecipitation on-beadTrp, On-beadLabel-free
HNF4A is required to specify glucocorticoid action in the liver2022PublicationChIPProtein GMammalian tissueManualN/AN/AN/AN/A
Proximity labelling identifies pro-migratory endocytic recycling cargo and machinery of the Rab4 and Rab11 families2022PublicationBioIDStreptavidinMammalian cellsManualDetergentGel-basedTrpLabel-free
Building Spectral Libraries from Narrow-Window Data-Independent Acquisition Mass Spectrometry Data2022PublicationDigest preparationAmineMammalian cellsAutomatedChaotropePrecipitation on-beadTrp, On-beadLabel-free
DirectMS1Quant: ultrafast quantitative proteomics with MS/MS-free mass spectrometry2022PublicationDigest preparationHILICBacterial cellsManualDetergentPrecipitation on-beadLysC, Trp, On-bead
Cyclin A and Cks1 promote kinase consensus switching to non-proline directed CDK1 phosphorylation2022PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualNativeReverse phaseTrp double digestIsobaric-tagging
Optimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT-based multiplexing2022PublicationDigest preparation, Phosphopeptide enrichmentHydroxyl, Ti-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadIsobaric-tagging
Plasma Membrane-Associated Proteins Identified in Arabidopsis Wild Type, lbr2-2 and bak1-4 Mutants Treated with LPSs from Pseudomonas syringae and Xanthomonas campestris2022PublicationDigest preparationHILICPlantManualDetergentHydrophilic affinityLysC, Trp, On-beadLabel-free
The IFT81-IFT74 complex enhances GTP hydrolysis to inactivate RabL2 during early steps of intraflagellar transport2022PublicationDigest preparationHILICProtein complexManualDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Localization of EccA3 at the growing pole in Mycobacterium smegmatis2022PublicationPeptide clean-upHILICBacterial cellsManualDetergentHydrophilic affinityTrp, On-beadLabel-free
ZAKβ is activated by cellular compression and mediates contraction-induced MAP kinase signaling in skeletal muscle2022PublicationDigest preparation, Phosphopeptide enrichmentAmine, Ti-IMACMammalian tissueAutomatedDetergentPrecipitation on-beadLysC, TrpLabel-free
Plasma proteome profiling of healthy individuals across the life span in a Sicilian cohort with long-lived individuals2022PublicationDigest preparationHILICBiofluidsManualDetergentHydrophilic affinityTrp, On-beadLabel-free
Phosphoproteomics of primary AML patient samples reveals rationale for AKT combination therapy and p53 context to overcome selinexor resistance2022PublicationDigest preparation, Phosphopeptide enrichmentAmine, Ti-IMACMammalian tissueAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
PROBER identifies proteins associated with programmable sequence-specific DNA in living cells2022PublicationBioIDStreptavidinMammalian cellsAutomatedDetergentAffinity captureTrpLabel-free
The Plasmodium falciparum Nuclear Protein Phosphatase NIF4 Is Required for Efficient Merozoite Invasion and Regulates Artemisinin Sensitivity2022PublicationPhosphopeptide enrichmentTi-IMACPlasmodiumManualChaotropeIn-solutionTrpIsobaric-tagging
Triple-threat quantitative multiplexed plasma proteomics analysis on immune complex disease MRL-lpr mice2022PublicationAffinity captureProtein GBiofluidsManualNativeReverse phaseLysC, TrpIsobaric-tagging
Biochemically validated structural model of the 15-subunit IFT-B complex2022PublicationDigest preparationHILICProtein complexManualDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Galectin-1 upregulates IDO1 and PD-L1 and induces a tolerogenic tumor-associated macrophage phenotype2022PublicationDigest preparation, Phosphopeptide enrichmentAmine, Ti-IMACMammalian tissueAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadIsobaric-tagging
IQGAP1 and RNA Splicing in the Context of Head and Neck via Phosphoproteomics2022PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualChaotropePrecipitation in-solutionLysC, TrpIsobaric-tagging
Fisetin induces DNA double-strand break and interferes with the repair of radiation-induced damage to radiosensitize triple negative breast cancer cells2022PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualChaotropePrecipitation in-solutionTrpMetabolic labelling
Rapid, semi-automated immunopeptidome profiling2022Application noteImmunopeptidomicsProtein A MAXMammalian cellsAutomatedDetergentOffline desaltN/ALabel-free
ABP1–TMK auxin perception for global phosphorylation and auxin canalization2022PublicationPhosphopeptide enrichmentTi-IMACPlantManualDetergentFilter-basedTrpMetabolic labelling
Quantitative phosphoproteome analysis of Streptomyces coelicolor by immobilized zirconium (IV) affinity chromatography and mass spectrometry reveals novel regulated protein phosphorylation sites and sequence motifs2022PublicationPhosphopeptide enrichmentZr-IMACBacterial cellsAutomatedChaotropePrecipitation in-solutionLysC, TrpMetabolic labelling
Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover2022PublicationDigest preparationAmineYeast cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organization2022PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentPrecipitation in-solutionTrpIsobaric-tagging
Activity-Based Proteomic Identification of the S-Thiolation Targets of Ajoene in MDA-MB-231 Breast Cancer Cells2022PublicationAffinity captureStreptavidinMammalian cellsManualNativeAffinity captureTrpLabel-free
Plasmodium falciparum CRK4 links early mitotic events to the onset of S-phase during schizogony2022PublicationAffinity captureStreptavidinPlasmodiumManualDetergentOnline desaltTrp, On-beadLabel-free
ABP1–TMK auxin perception for global phosphorylation and auxin canalization2022PublicationPhosphopeptide enrichmentTi-IMACPlantManualDetergentFilter-basedTrpMetabolic labelling
Quantitative phosphoproteome analysis of Streptomyces coelicolor by immobilized zirconium (IV) affinity chromatography and mass spectrometry reveals novel regulated protein phosphorylation sites and sequence motifs2022PublicationPhosphopeptide enrichmentTi-IMACBacterial cellsAutomatedChaotropePrecipitation in-solutionLysC, TrpMetabolic labelling
Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover2022PublicationDigest preparationAmineYeast cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organization2022PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentPrecipitation in-solutionTrpIsobaric-tagging
Activity-Based Proteomic Identification of the S-Thiolation Targets of Ajoene in MDA-MB-231 Breast Cancer Cells2022PublicationAffinity captureStreptavidinMammalian cellsManualNativeAffinity captureTrp, On-beadLabel-free
Biochemically validated structural model of the 15-subunit intraflagellar transport complex IFT-B2022PublicationDigest preparationHILICProtein complexManualDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Choice of High-Throughput Proteomics Method Affects Data Integration with Transcriptomics and the Potential Use in Biomarker Discovery2022PublicationDigest preparationHILICMammalian tissueAutomatedDetergentHydrophilic affinityTrp, On-beadLabel-free
Adjuvants in fungicide formulations can be skin sensitizers and cause different types of cell stress responses2022PublicationDigest preparationHILICMammalian cellsAutomatedDetergentHydrophilic affinityTrp, On-beadLabel-free
Differential ultracentrifugation enables deep plasma proteomics through enrichment of extracellular vesicles2022PublicationEnrichment of extracellular vesiclesHydroxylBiofluidsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Functional annotation of proteins for signaling network inference in non-model species2022PublicationPhosphopeptide enrichmentTi-IMACPlantManualDetergentPrecipitation in-solutionLysC, TrpLabel-free
Characterization of TGF-? signaling in a human organotypic skin model reveals that loss of TGF-?RII induces invasive tissue growth2022PublicationDigest preparation, Phosphopeptide enrichmentAmine, Ti-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
CLK1/CLK2-driven signalling at the Leishmania kinetochore is captured by spatially referenced proximity phosphoproteomic2022PublicationBioID, Phosphopeptide enrichmentStreptavidin, Ti-IMACLeishmania protozoaManualDetergentAffinity captureLysC, Trp, On-beadLabel-free
Functional diversification despite structural congruence in the HipBST toxin-antitoxin system of Legionella pneumophila2022PublicationPhosphopeptide enrichmentTiO2Bacterial cellsManualDetergentOffline desaltLysC, TrpNon isobaric-tagging
An ultra-fast, proteome-wide response to the plant hormone auxin2022Ti-IMACPhosphopeptide enrichmentTi-IMACPlantManualChaotropePrecipitation in-solutionTrpLabel-free
Dynamic Data Independent Acquisition Mass Spectrometry with Real-Time Retrospective Alignment2022PublicationDigest preparationHydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadTrp, On-beadMetabolic labelling
RAPID & SCALABLE OFF-LINE PEPTIDE FRACTIONATION ON ZWITTERIONIC MAGNETIC MICROPARTICLES2022PosterPeptide fractionationHILICMammalian cellsAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
Hybrid-DIA: Intelligent Data Acquisition for Simultaneous Targeted and Discovery Phosphoproteomics in Single Spheroids2022PublicationDigest preparation, Phosphopeptide enrichmentHydroxyl, Ti-IMAC, Zr-IMACSpheroidsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadHybrid DIA-PRM
Temporal phosphoproteomics reveals WEE1-dependent control of 53BP1 pathway2022PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualChaotropeOffline desaltLysC, TrpIsobaric-tagging
Phosphoproteomic Analysis of Dopamine D2 Receptor Signaling Reveals Interplay of G Protein- and ?-Arrestin-Mediated Effects2022PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualOrganicOffline desaltTrpLabel-free
SP3 Protocol for Proteomic Plant Sample Preparation Prior LC-MS/MS2022PublicationDigest preparationHILICPlantManualDetergentHydrophilic affinityTrp, On-beadLabel-free
A proteomics dataset capturing myeloid cell responses upon cellular exposure to fungicides, 2023PublicationDigest preparationHILICMammalian cellsAutomatedDetergentHydrophilic affinityTrp, On-beadLabel-free
Characterisation of Colorectal Cancer Cell Lines through Proteomic Profiling of Their Extracellular Vesicles2023PublicationEnrichment of extracellular vesicles, Phosphopeptide enrichmentHILIC, Ti-IMACMammalian cellsManualDetergentPrecipitation on-beadTrp, On-beadLabel-free
Glucose dissociates DDX21 dimers to regulate mRNA splicing and tissue differentiation2023PublicationBioIDStreptavidinMammalian cellsManualDetergentAffinity captureTrp, On-beadLabel-free
A TREM2-activating antibody with a blood–brain barrier transport vehicle enhances microglial metabolism in Alzheimer’s disease models2023PublicationBioIDStreptavidinMammalian cellsManualDetergentAffinity captureN/ALabel-free
Mass Spectrometry-Based Proteomics Workflows in Cancer Research: The Relevance of Choosing the Right Steps2023Review publicationCancer research, Phosphopeptide enrichmentTi-IMAC
Signaling Network Response to Alpha-Particle Targeted Therapy with Actinium-225 Labeled Minigastrin Analogue 225Ac-PP-F11N Reveals Radiosensitizing potential of HDAC inhibitors2023PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueAutomatedChaotropeFilter-basedTrpLabel-free
Enzymatic and proteomic exploration into the inhibitory activities of lemongrass and lemon essential oils against Botrytis cinerea (causative pathogen of gray mold)2023PublicationDigest preparationHILICPlantManualChaotropeHydrophilic affinityTrp, On-beadLabel-free
SUMO Proteomics Analyses Identify Protein Inhibitor of Activated STAT-Mediated Regulatory Networks Involved in Cell Cycle and Cell Proliferation2023PublicationSUMO peptide enrichmentProtein AMammalian cellsManualChaotropeAffinity captureN/ALabel-free
Optimization of Instrument Parameters for Efficient Phosphopeptide Identification and Localization by Data-dependent Analysis Using Orbitrap Tribrid Mass Spectrometers2023PublicationPhosphopeptide enrichmentTi-IMAC, Zr-IMACMammalian tissueAutomatedDetergentPrecipitation on-beadTrpLabel-free
Synthetic Siglec-9 Agonists Inhibit Neutrophil Activation Associated with COVID-192021PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualDetergentPrecipitation on-filterTrpIsobaric-tagging
Modulation of immune cell reactivity with cis-binding Siglec agonists2021PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualDetergentPrecipitation on-filterTrpIsobaric-tagging
ARF-family global interactome mapping uncovers spatial organization of cellular signaling pathways2023PublicationAffinity captureHydroxylMammalian cellsManualDetergentPrecipitation on-beadTrp, On-beadLabel-free
Pptc7 maintains mitochondrial protein content by suppressing receptor-mediated mitophagy2023PublicationPhosphopeptide enrichmentTiO2Mammalian tissueManualChaotropeOffline desaltLysC, TrpIsobaric-tagging
Proteomic analysis identifies dysregulated proteins and associated molecular pathways in a cohort of gallbladder cancer 2023PublicationDigest preparationHydroxylMammalian tissue, BiofluidsAutomatedDetergent, ChaotropePrecipitation on-bead, Hydrophilic affinityLysC, Trp, On-beadLabel-free
Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS2023PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualChaotropePrecipitation in-solutionTrpLabel-free
Pulmonary maternal immune activation does not extend through the placenta but leads to fetal metabolic adaptation2023PublicationDigest preparation, Phosphopeptide enrichmentHILIC, Ti-IMACMammalian tissueAutomatedDetergentHydrophilic affinityTrp, On-beadIsobaric-tagging
Proximity mapping of desmosomes reveals a striking shift in their molecular neighbourhood associated with maturation2023PublicationBioIDStreptavidinMammalian cellsManualDetergentGel-basedTrpLabel-free
Ki-67 is necessary during DNA replication for forks protection and genome stability2023PublicationPhosphopeptide enrichment Ti-IMACMammalian tissueManualDetergentGel-basedTrpMetabolic labelling
AKT activity orchestrates marginal zone B cell development in mice and humans2023PublicationPhosphopeptide enrichmentZr-IMACMammalian tissueManualChaotropePrecipitation on-filterTrpLabel-free
In vitro and ex vivo proteomics of Mycobacterium marinum biofilms and the development of biofilm-binding synthetic nanobodiess2023PublicationDigest preparationAmineBacterial cellsManualDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Dual agonistic and antagonistic roles of ZC3H18 provides for co-activation of distinct nuclear RNA decay pathways2023PublicationDigest preparationHILICMammalian cellsManualDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Modular, scalable and automatable on-beads pipeline for bottom-up proteome and phosphoproteome profiling with built-in peptide and phosphopeptide fractionation2023PosterPhosphopeptide fractionation, Peptide fractionationHILICMammalian cellsAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
Automated sample preparation for bottom-up urinary proteome profiling in clinical proteomics2023PosterUrine proteomicsHILICBiofluidsAutomatedChaotrope, DetergentHydrophilic affinityTrp, On-beadLabel-free
SAPrIm, a semi-automated protocol for mid-throughput immunopeptidomics2023PublicationImmunopeptidomicsProtein AMammalian cellsAutomatedDetergentOffline desaltN/ALabel-free
Microscale proximity-dependent biotinylation procedure for low cell input samples using protease-resistant streptavidin on a magnetic substrate2023PosterBioIDStreptavidin MSMammalian cellsManualDetergentAffinity captureTrp, On-beadLabel-free
MagReSyn® Streptavidin MS: enabling more sensitive affinity workflows2023Application noteAffinity capture, BioIDStreptavidinYeast cellsAutomatedDetergentAffinity captureTrp, On-beadLabel-free
Evaluating the performance of the Astral mass analyzer for quantitative proteomics using data independent acquisition 2023PublicationDeep plasma profiling, Enrichment of extracellular vesiclesSAXBiofluidsAutomatedNativePrecipitation on-beadTrp, On-beadLabel-free
Mag-Net: Rapid enrichment of membrane-bound particles enables high coverage quantitative analysis of the plasma proteome2024PublicationDeep plasma profiling, Enrichment of extracellular vesiclesSAXBiofluidsAutomatedNativePrecipitation on-beadTrp, On-beadLabel-free
Quantitative phosphoproteomics reveals novel roles of cAMP2023PublicationPhosphopeptide enrichmentTi-IMACPlantManualDetergent, OrganicFilter-basedTrpLabel-free
A complete and automated end-to-end sample preparation strategy for high-throughput and standardized proteomics with high sensitivity 2023PosterDigest preparationHydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
Mag-Net, Enrichment of membrane-bound vesicles from Plasma using MagReSyn® SAX for analysis by LC-MSMS2023ProtocolDeep plasma profiling, Enrichment of extracellular vesiclesSAXBiofluidsAutomatedNativePrecipitation on-beadTrp, On-beadLabel-free
The soybean immune receptor GmBIR1 regulates host transcriptome, spliceome, and immunity during cyst nematode infection2023PublicationPhosphopeptide enrichmentZr-IMAC, ZrO2PlantManualDetergent, OrganicPrecipitation in-solutionTrpLabel-free
ARS2 instructs early transcription termination-coupled RNA decay by recruiting ZC3H4 to nascent transcripts2023PublicationDigest preparationHILICMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Quantification of proteomic profile changes in the hemolymph of crayfish during in vitro coagulation2023PublicationDigest preparationHILICCrustaceansAutomatedDetergentHydrophilic affinityLysC, Trp, On-beadLabel-free
Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme research2023PublicationPhosphopeptide enrichmentTi-IMACBacterial cellsAutomatedDetergent, OrganicGel-basedTrpLabel-free
Calcineurin regulates aldosterone production via dephosphorylation of NFATc42023PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsAutomatedDetergent, OrganicFilter-basedTrpLabel-free
DYRK3 enables secretory trafficking by maintaining the liquid-like state of ER exit sites2023PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsAutomatedNativeAffinity captureTrp, On-beadLabel-free
Custom Workflow for the Confident Identification of Sulfotyrosine-Containing Peptides and Their Discrimination from Phosphopeptides2023PublicationSulfopeptide enrichmentZr-IMACMammalian cellsManualDetergentPrecipitation on-beadTrpLabel-free
The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport2023PublicationDigest preparationHILICAlgaeAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Functional annotation of proteins for signaling network inference in non-model species2023PublicationPhosphopeptide enrichmentTi-IMACPlantManualNativePrecipitation in-solutionLysC, TrpLabel-free
Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling2023PublicationDigest preparation, Phosphopeptide enrichmentHydroxyl, Ti-IMAC, Zr-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free, Isobaric-tagging
IMBAS-MS discovers organ-specific HLA peptide patterns in plasma2023PublicationImmunopeptidomicsStreptavidinPlasmaAutomatedDetergentNativeAutomated desaltingLabel-free
Urine-HILIC: Automated Sample Preparation for Bottom-Up Urinary Proteome Profiling in Clinical Proteomics2023PublicationUrine proteomicsHILICBiofluidsAutomatedChaotrope, DetergentHydrophilic affinityTrp, On-beadLabel-free
Phosphorylation-controlled cohesion of a nuclear condensate regulates mRNA retention2023PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
Rewiring of the phosphoproteome executes two meiotic divisions2023PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentOffline desaltTrpIsobaric-tagging
Atypical cofilin signaling drives dendritic cell migration through the extracellular matrix via nuclear deformation2024PublicationDigest preparation, Phosphopeptide enrichmentTi-IMAC, AmineMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
RNA binding proteins and glycoRNAs form domains on the cell surface for cell penetrating peptide entry2023PublicationAffinity captureStreptavidinMammalian cellsManualNativePrecipitation on-filterTrpLabel-free
Clinical proteomics reveals vulnerabilities in non-invasive breast ductal carcinoma and drives personalized treatment strategies2023PublicationDigest preparationHydroxylFFPEManualDetergentPrecipitation on-beadTrp, On-beadLabel-free
Spatiotemporal Proteomics Deciphers Functional Selectivity of EGFR ligands2023PublicationDigest preparation, Phosphopeptide enrichmentTi-IMAC, HydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
A BRCA2 germline mutation and high expression of immune checkpoints in a TNBC patient2023PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueAutomatedDetergentOffline desaltTrpLabel-free
GLP-1R signaling neighborhoods associate with the susceptibility to adverse drug reactions of incretin mimetics2023PublicationDigest preparation, Phosphopeptide enrichmentTiO2, AmineMammalian cellsManualDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Wnt/β-catenin and NFκB signaling synergize to trigger growth-factor-free regeneration of adult primary human hepatocytes2023PublicationPhosphopeptide enrichmentTiO2SpheroidsManualDetergentPrecipitation LysC, TrpIsobaric-tagging
Autoantibody binding and unique enzyme-substrate intermediate conformation of human transglutaminase 32023PublicationDigest preparationAmineRecombinant proteinManualNativePrecipitation on-beadTrp, On-beadLabel-free
Casein kinase 2 activity is a host restriction factor for AAV transduction2023PublicationPhosphopeptide enrichmentTiO2Mammalian cellsManualDetergentOffline desaltLysC, TrpLabel-free
Phosphoproteome analyses pinpoint the F-box protein SLOW MOTION as a regulator of warm temperature-mediated hypocotyl growth in Arabidopsis2023PublicationPhosphopeptide enrichmentTi-IMACPlantManualDetergentPrecipitation in-solutionLysC, TrpLabel-free
HSF-1 promotes longevity through ubiquilin-1 dependent mitochondrial network remodelling2023PublicationDigest preparationHydroxylC. elegansAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
Comprehensive Overview of Bottom-up Proteomics using Mass Spectrometry2023Review publicationPhosphopeptide enrichmentTi-IMAC, Zr-IMAC
One-Hour Phosphoproteome Analysis with the Orbitrap Astral Mass Spectrometer2023PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualChaotrope, DetergentOffline desaltLysC, TrpLabel-free
Narrow-window DIA: Ultra-fast quantitative analysis of comprehensive proteomes with high sequencing depth2023PublicationDigest preparationHydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
Dynamic Protein Phosphorylation in Streptococcus pyogenes during Growth, Stationary Phase, and Starvation2024PublicationPhosphopeptide enrichmentTi-IMAC, TiO2Bacterial cellsManualDetergentPrecipitation in-solutionTrpLabel-free
Fully automated workflow for integrated sample digestion and Evotip loading enabling high-throughput clinical proteomics2023PublicationDigest preparation, Phosphopeptide enrichmentHydroxyl, Ti-IMACMammalian tissue, Mammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
A chromatin-regulated biphasic circuit coordinates IL-1β-mediated inflammation2020PublicationChIPProtein A, Protein GMammalian cellsManualN/AN/AN/AqPCR
Fast and Deep Phosphoproteome Analysis with the Orbitrap Astral Mass Spectrometer2024PublicationPhosphopeptide enrichmentTi-IMACMammalian tissue, Mammalian cellsManualChaotrope, DetergentOffline desaltLysC, TrpLabel-free
Immunopeptidomics in the Era of Single-Cell Proteomics2023ReviewImmunopeptidomicsProtein AN/AN/AN/AN/AN/AN/A
High-throughput proteomics uncovers exercise training and type 2 diabetes–induced changes in human white adipose tissue2023PublicationDigest preparationHydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
p38γ/δ activation alters cardiac electrical activity and predisposes to ventricular arrhythmia2023PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueManualDetergentFilter-basedTrpIsobaric-tagging
High-throughput proteomics uncovers exercise training and type 2 diabetes–induced changes in human white adipose tissue2023PublicationDigest preparationHydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Mapping cardiac remodeling in chronic kidney disease2023PublicationDigest preparation, Phosphopeptide enrichmentHydroxyl, Ti-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Secretomics reveals hormone-therapy of breast cancer may induce survival by facilitating hypercoagulation and immunomodulation in vitro2024PublicationDigest preparationHILICBiofluidsAutomatedDetergentHydrophilic affinityTrp, On-beadLabel-free
Phosphorylation-controlled cohesion of a nuclear condensate regulates mRNA retention2023PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsAutomatedDetergentAffinity captureTrp, On-beadLabel-free
Delineation of signaling routes that underlie differences in macrophage phenotypic states2023PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsAutomatedDetergentFilter-basedTrpLabel-free
Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor formation and progression 2024PublicationDigest preparation, Phosphopeptide enrichmentHydroxyl, Zr-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Systematic optimization of automated phosphopeptide enrichment for high-sensitivity phosphoproteomics 2024PublicationDigest preparation, Phosphopeptide enrichmentHydroxyl, Zr-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
End-to-end fully automated digestion protocol on the OT-22024Application noteDigest preparationHydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Unlocking the plasma proteome with Evotip Pure2024Application noteDeep plasma profilingSAXBiofluidsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Deep Plasma Profiling using Mag-Net: Maximizing Proteome Depth2024PosterDeep plasma profilingSAXBiofluidsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistance2024PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadIsobaric-tagging
Deciphering molecular mechanisms of synergistic growth reduction in kinase inhibitor combinations2024PublicationDigest preparation, Phosphopeptide enrichmentHydroxyl, Ti-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Phosphoproteomic Analysis and Organotypic Cultures for the Study of Signaling Pathways2024PublicationDigest preparation, Phosphopeptide enrichmentTi-IMAC, Amine, HydroxylOrganiodsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
PRMT1 acts as a suppressor of MHC-I and anti-tumor immunity2024PublicationImmunopeptidomicsProtein AMammalian cellsAutomatedDetergentOffline desaltN/ALabel-free
Phosphorylation of the alpha-I motif in SYMRK drives root nodule organogenesis2024PublicationPhosphopeptide enrichmentZr-IMACRecombinant proteinManualDetergentReverse phaseLysC, Trp, On-beadLabel-free
BPTF cooperates with MYCN and MYC to link neuroblastoma cell cycle control to epigenetic cellular states2024PublicationDigest preparationHydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Streamlined proteome-wide identification of drug targets indicates organ-specific engagement2024PublicationDigest preparationHydroxylMammalian tissueManualDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Personalized Molecular Signatures of Insulin Resistance and Type 2 Diabetes2024PublicationDigest preparation, Phosphopeptide enrichmentTi-IMAC, Zr-IMAC, HydroxylMammalian tissueAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Inhibition of the Complement Pathway Induces Cellular Proliferation and Migration in Pancreatic Ductal Adenocarcinoma2024PublicationDigest preparationHILICMammalian tissueAutomatedDetergentHydrophilic affinityLysC, Trp, On-beadLabel-free
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition2024PublicationDigest preparationAmineMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Systematic optimization of automated phosphopeptide enrichment for high-sensitivity phosphoproteomics2024PublicationDigest preparation, Phosphopeptide enrichmentZr-IMAC, HydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
High-throughput proteomics uncovers exercise training and type 2 diabetes–induced changes in human white adipose tissue2024PublicationDigest preparationHydroxylMammalian tissueAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Evaluation of PAC and FASP Performance: DIA-Based Quantitative Proteomic Analysis2024PublicationDigest preparationHydroxylMammalian cellsManualDetergentPrecipitation on-beadTrp, On-beadLabel-free
The cAMP-dependent phosphorylation footprint in response to heat stress2024PublicationPhosphopeptide enrichmentTi-IMACPlantManualOrganicPrecipitationLysC, TrpLabel-free
Soybean MKK2 establishes intricate signalling pathways to regulate soybean response to cyst nematode infection2024PublicationPhosphopeptide enrichmentZrO2, Zr-IMACPlantManualNAPrecipitationLysC, TrpLabel-free
Multi-omic analyses reveal a differential contribution of chromatin-associated PP1 holoenzymes to mitotic exit and G1 re-establishment2024PublicationPhosphopeptide enrichmentTi-IMACMammalian cellsManualDetergentFilter-basedTrpLabel-free
A Semiautomated Proteomics and Phosphoproteomics Protocol for the Identification of Novel Therapeutic Targets and Predictive Biomarkers in In Vivo Xenograft Models of Pediatric Cancers2024PublicationPhosphopeptide enrichmentZr-IMACXenograftsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
O-linked glycosylations in human milk casein and major whey proteins during lactation2024PublicationO-linked glycan enrichmentZrO2, TiO2BiofluidsAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
A framework for quality control in quantitative proteomics2024PublicationDigest preparationHydroxyl, CarboxylBiofluidsAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
PBK/TOPK mediates Ikaros, Aiolos and CTCF displacement from mitotic chromosomes and alters chromatin accessibility at selected C2H2-zinc finger protein binding sites2024PublicationDigest preparation, Affinity captureHydroxyl, Protein GMammalian cellsManualDetergentPrecipitation on-beadTrp, On-beadLabel-free
Multiomics Analysis of PCB126’s Effect on a Mouse Chronic-Binge Alcohol Feeding Model2024PublicationPhosphopeptide enrichmentTi-IMACMammalian tissueAutomatedDetergentPrecipitation on-filterLysC, TrpLabel-free
Phosphoproteomic investigation of targets of protein phosphatases in EGFR signaling2024PublicationDigest preparation, Phosphopeptide enrichmentHydroxyl, Ti-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Cheap, Robust and Versatile Two-Dimensional Chromatography system for Proteomics of Nanogram Scale Samples2024PublicationPhosphopeptide enrichmentZr-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Digging deeper into the plasma proteome using Mag-Net enrichment and Zeno SWATH data-independent acquisition (DIA)2024Application noteDeep plasma profiling, Enrichment of extracellular vesiclesSAXBiofluidsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Systematic optimization of automated phosphopeptide enrichment for high-sensitivity phosphoproteomics 2024PublicationDigest preparation, Phosphopeptide enrichmentHydroxyl, Zr-IMACMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Mapping the global interactome of the ARF family reveals spatial organization in cellular signaling pathways2024PublicationDigest preparationHydroxylMammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
SMARCAL1 ubiquitylation controls its association with RPA-coated ssDNA and promotes replication fork stability2024PublicationAffinity captureStreptavidinMammalian cellsManualNativeAffinity captureTrp, On-beadLabel-free
Refinement of paramagnetic bead-based digestion protocol for automatic sample preparation using an artificial neural network2024PublicationDigest preparationHydroxylMammalian cells, Mammalian tissue, Plant, BiofluidsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Development of highly multiplex targeted proteomics assays in biofluids using the Stellar mass spectrometer2024PublicationDeep plasma profiling, Enrichment of extracellular vesicles, Digest preparationHydroxyl, SAXBiofluidsAutomatedNativePrecipitation on-beadTrp, On-beadLabel-free
Comparison of the Proteome of Huh7 Cells Transfected with Hepatitis B Virus Subgenotype A1, with or without G1862T2024PublicationDigest preparationHILICMammalian cellsAutomatedDetergentPrecipitation on-beadTrp, On-beadLabel-free
Unravelling biological processess and EGFR pathway regulation by the receptor-like protein tyrosine phosphatase PTPRH in non-small cell lung cancer2024PublicationBioIDStreptavidinMammalian cellsManualDetergentAffinity captureTrypsinLabel-free
Time-based quantitative proteomic and phosphoproteomic analysis of A549-ACE2 cells during SARS-CoV-2 infection2024PublicationDigest preparationAmineBiofluidsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadIsobaric-tagging
EPLINα controls integrin recycling from Rab21 endosomes to drive breast cancer cell migration2024PublicationBioIDStreptavidinMammalian cellsManualDetergentAffinity captureTry (in-gel)Label-free
Cardiac myosin binding protein-C phosphorylation as a function of multiple protein kinase and phosphatase activities2024PublicationPhosphopeptide enrichmentTiO2Mammalian tissueManualDetergentGel-basedTrp (in-gel)Label-free
An automated sample preparation workflow for proteomic profiling of membrane-bound vesicls from urine2024PosterEnrichment of extracellular vesicles, Urine proteomicsSAX, HILIC, HydroxylBiofluidAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Isolation of plasma-derived extracellular vesicles using the Mag-Net method to identify potential protein signatures for the diagnosis of pancreatic ductal adenocarcinoma2024PosterDeep plasma profiling, Enrichment of extracellular vesiclesHILIC, SAXBiofluidAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Inter-lab assessment of standardized and fully automated workflows integrated with Evosep One2024PosterDeep plasma profiling, Enrichment of extracellular vesicles, Digest preparationHydroxyl, SAXBiofluid, Mammalian cellsAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Calcium-triggered (de)ubiquitination events in synapses2024PublicationPhosphopeptide enrichmentZr-IMACMammalian cellsManualOrganicReverse phaseLysC, TrpIsobaric tagging
Repurposed 3D Printer Allows Economical and Programmable Fraction Collection for Proteomics of Nanogram Scale Samples2024PublicationPhosphopeptide enrichmentZr-IMACCommercial cell lysateAutomatedNAPrecipitation on-beadLysC, TrpLabel-free
An E3 Ubiquitin Ligase Localization Screen Uncovers DTX2 as a Novel ADP-Ribosylation-Dependent Regulator of DNA Double-Strand Break Repair2024PublicationAffinity captureStreptavidinMammalian cellsManualDetergentGel-basedN/AN/A
Proteomic analysis identifies dysregulated proteins in albuminuria: a South African pilot study2024PublicationDigest preparation, Urine proteomicsHILICBiofluidAutomatedDetergentPrecipitation on-beadTrypsinLabel-free
Data-independent LC-MS/MS analysis of ME/CFS plasma reveals a dysregulated coagulation system, endothelial dysfunction, downregulation of complement machinery2024PublicationDigest preparationHILICBiofluidManualNAHydrophilic affinityTrp, On-beadLabel-free
Unraveling the differential mechanisms of revascularization promoted by MSCs & ECFCs from adipose tissue or umbilical cord in a murine model of critical limb-threatening ischemia2024PublicationDigest preparationAmineMammalian tissueAutomatedDetergentPrecipitation on-beadLysC, Trp, On-beadLabel-free
Lysine Phoshoglycerylation Is Widespread in Bacteria and Overlaps with Acylation2024PublicationPhosphopeptide enrichmentTi-IMAC, TiO2Bacterial cellsManualDetergentPrecipitation in solutionTrpLabel-free
FAS Inhibited Proteomics and Phosphoproteomics Profiling of Colorectal Cancer Spheroids Shows Activation of Ferroptotic Death Mechanism2024PublicationPhosphopeptide enrichmentTi-IMACSpheroidsManualDetergentPrecipitation on-filterTrp, LysCLabel-free
High-Throughput Proteomics and Phosphoproteomics of Rat Tissues Using Microflow Zeno SWATH2024PublicationPhosphopeptide enrichmentTi-IMAC, Zr-IMACMammalian tissue, Rat organsManualDetergentMixed mode SPETrp, LysCLabel-free
Quantitative Comparison of Deparaffinization, Rehydration, and Extraction Methods for FFPE Tissue Proteomics and Phosphoproteomics2024PublicationPhosphopeptide enrichmentTi-IMAC, Zr-IMACFFPEManualDetergentComparisonTrp, LysCLabel-free
Environmental fluoxetine promotes skin cell proliferation and wound healingP2024PublicationPhosphopeptide enrichmentZr-IMACMammalian cellsManualChaotropeOnline desaltTrpLabel-free
Co-opting templated aggregation to degrade pathogenic tau assemblies and improve motor function2024PublicationDigest preparationHydroxylMammalian tissueAutomatedSarkosyl insoluble extractionPrecipitation on-beadTrpLabel-free
Cryo-electron tomography reveals how COPII assembles on cargo-containing membranes2024PublicationDigest preparationHydroxylYeast cellsManualNativePrecipitation on-beadTrpLabel-free

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