For covalent ligand immobilization via their primary amine residues
MagReSyn® Carboxyl is a magnetic polymeric microparticles support designed for covalent immobilization of primary amine-containing ligands through carbodiimide-succinimidyl ester activation and subsequent amide bond formation. Various strategies for the activation of carboxylate particles have been documented, but the most commonly used method for coupling proteins and other primary amine-containing ligands is through activation by EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide) in an aqueous reaction. This reaction can be either single-step or a two-step reaction requiring the addition of N-hydroxysuccinimide (NHS) or the more stable sulfo-NHS yielding NHS ester or sulfo NHS ester intermediates.
Please note that coupling of proteins through NHS-EDC activation can be tricky, requires fresh reagents, and is prone to interference from a range of routine buffers used in life-sciences. For simpler coupling of your ligand we would recommend using MagReSyn® Amine with glutaraldehyde or epoxide activation.
Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Binding capacity: > 20 mg.ml-1 (BSA)
Bead size: ~5-10 µm
Formulation: 20 mg.ml-1 suspension in 20% ethanol
MagReSyn® Carboxyl Performance Superiority
Performance superiority of MagReSyn® Carboxyl over the leading suppliers’ supports.
Green bar represents MagReSyn® Carboxyl, orange bars represent alternate suppliers.
Values used for alternate products are the respective suppliers’ claimed capacities.
NOTE: Activation of carboxyl by NHS-EDC for coupling of proteins is not trivial.
Coupling is subject to interference by a range of contaminants, we therefore recommend you
consult with a suitable reference such as Bioconjugate Techniques prior to experimentation.
As an alternate approach we recommend epoxide or glutaraldehyde activation of MagReSyn® Amine.
TERMS AND CONDITIONS
Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.
Citations and References
The co-immobilization of P450-type nitric oxide reductase and glucose dehydrogenase for the continuous reduction of nitric oxide via cofactor recycling
– Seike Garny et al.
Enzyme & Microbial Technology 85 (2015)
Ultrasensitive proteome analysis using paramagnetic bead technology
– Christopher S. Hughes et al.
Molecular Systems Biology 10 (2014)