NEW: MagReSyn® Ti-IMAC HP
Magnetic microparticles with chelated Ti4+ metal ions for highly-specific phosphopeptide enrichment
Protein phosphorylation is one of the most important post-translational modifications and is a critical process in cellular signaling and regulation of cellular networks. Comprehensive analysis of the phosphoproteome is a challenging task due to the transient and sub-stoichiometric nature of phosphorylation sites. High-throughput phosphoproteome analysis by mass spectrometry requires compatible technologies than can specifically enrich phosphopeptides. MagReSyn® Ti-IMAC microparticle have a flexible linker (to reduce steric hindrance) activated with phosphonate groups for Ti4+ chelation. The unique properties of the proprietary ReSyn microparticle technology allows extremely specific, reproducible enrichment of phosphopeptides from complex biological samples/protein digests. The microparticles can be used either alone, or in combination with MagReSyn® TiO2, MagReSyn® Zr-IMAC and/or MagReSyn® ZrO2 to increase phosphoproteome coverage.
The new High Performance (HP) version of our popular titanium IMAC for phosphopeptide enrichment, was externally validated by the Olsen Lab, and the use first published in Nature Communications, by Dorte B Bekker Jensen et al., 2020. The product offers potentially increased recovery and sample coverage, with application to low-quantity peptide input.
Each batch of product for phosphopeptide enrichment is validated for the application using our stringent mass spectrometry based QC procedures to ensure maximum reproducibility.
IMPORTANT NOTE: This product has a limited shelf-life of 6 months due the instability of the chelated Titanium ions, please consider carefully how much product is required. Zr-IMAC & Zr-IMAC HP offer similar coverage for most sample types, and we would recommend evaluation of these products for low throughput applications.
Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Bead size: ~5-10 µm
Formulation: 20 mg.ml-1 suspension in 20% ethanol
MagReSyn® Ti-IMAC
Magnetic microparticles with chelated Ti4+ metal ions for highly-specific phosphopeptide enrichment
Our classic and highly published research tool for phosphopeptide enrichment, consisting of phosphonate groups chelated with Ti4+.
MagReSyn® Ti-IMAC has also been used in the development of new innovative sample preparation protocols. As an example Leutert et al., 2017, illustrated efficient enrichment of ADP-ribosylated peptides using Ti-IMAC, providing a comprehensive protocol in Methods in Molecular Biology.
Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Bead size: ~5-10 µm
Formulation: 20 mg.ml-1 suspension in 20% ethanol
Product Resources
TERMS AND CONDITIONS
Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.
Citations and References
Systems level analysis of time and stimuli specific signaling through PKA
– Michael Plank et al.
BioRxiv (2022)
MagReSyn® Ti-IMAC
Multi-omic analysis of the Arabidopsis clock activator mutant rve 4 6 8 reveals connections to carbohydrate metabolism and proteasome regulation
– S Scandola et al.
BioRxiv (2022)
MagReSyn® Ti-IMAC HP
Tissue-wide Effects Overrule Cell-intrinsic Gene Function in Cortical Projection Neuron Migration
– Andi H Hansen et al.
BioRxiv (2022)
MagReSyn® Ti-IMAC
STK25 inhibits PKA signaling by phosphorylating PRKAR1A
– Xiaokan Zhang et al.
BioRxiv (2022)
MagReSyn® Ti-IMAC & Zr-IMAC
Phosphoproteomic profiling of influenza virus entry reveals infection-triggered filopodia induction counteracted by dynamic cortactin phosphorylation
– Annika Hunziker et al.
Cell Reports (2022)
MagReSyn® Ti-IMAC
Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy
– Katharina I. Zittlau et al.
MCP (2022)
MagReSyn® Ti-IMAC
Unraveling virus-induced cellular signaling cascades by label-free quantitative phosphoproteomics
– Annika Hunziker & Silke Stertz
STAR Protocols (2022)
MagReSyn® Ti-IMAC
Glutamine-Fructose-6-Phosphate Transaminase 2 (GFPT2) Is Upregulated in Breast Epithelial–Mesenchymal Transition and Responds to Oxidative Stress
– Qiong Wang et al.
MCP (2022)
MagReSyn® Ti-IMAC
UDP-glucose dehydrogenase expression is upregulated following EMT and differentially affects intracellular glycerophosphocholine and acetylaspartate levels in breast mesenchymal cell lines.
– Qiong Wang et al.
Molecular Oncology (2021)
MagReSyn® Ti-IMAC
Plant Phosphoproteomics: Known Knowns, Known Unknowns, and Unknown Unknowns of an Emerging Systems Science Frontier
– Pratigya Subba et al.
OMICS (2021)
MagReSyn® Ti-IMAC
Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution
– Ana Martinez-Val et al.
Nature Communications (2021)
MagReSyn® Amine, MagReSyn® Ti-IMAC HP
Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion
– Gaëlle Lentini et al.
Nature Communications (2021)
MagReSyn® Ti-IMAC
Modulation of immune cell reactivity with cis-binding Siglec agonists
– Corleone S. Delaveris et al.
PNAS (2021)
MagReSyn® Ti-IMAC
Unraveling the MAX2 Protein Network in Arabidopsis thaliana: Identification of the Protein Phosphatase PAPP5 as a Novel MAX2 Interactor
– Sylwia Struk et al.
MCP (2021)
MagReSyn® Ti-IMAC
The membrane-localized protein kinase MAP4K4/TOT3 regulates thermomorphogenesis
– Lam Dai Vu et al.
Nature Communications (2021)
MagReSyn® Ti-IMAC
Proteomics of resistance to Notch1 inhibition in acute lymphoblastic leukemia reveals targetable kinase signatures
– Giulia Franciosa et al.
Nature Communications (2021)
MagReSyn® Ti-IMAC HP & Amine
Multi-omic analysis reveals the biochemical changes underpinning the varied phenotypes of the Arabidopsis long-period mutant rve 4 6 8
– S Scandola et al.
BioRxiv (2021)
MagReSyn® Ti-IMAC HP
Molecular roadmap of the healthy stress response in the mouse hippocampus
– Lukas M. von Ziegler et al.
BioRxiv (2021)
MagReSyn® Ti-IMAC
Rapid auxin-mediated phosphorylation of Myosin regulates trafficking and polarity in Arabidopsis
– Huibin Han et al.
BioRxiv (2021)
MagReSyn® Ti-IMAC
Identifying cytokine signaling signatures in primary human Th-1 cells by phospho-proteomics analysis
– Jonathan Martinez-Fabregas et al.
STAR Protocols (2021)
MagReSyn® Ti-IMAC
Competitive binding of STATs to receptor phospho-Tyr motifs accounts for altered cytokine responses
– Stephan Wilmes et al.
eLife (2021)
MagReSyn® Ti-IMAC
CDK8 fine-tunes IL-6 transcriptional activities by limiting STAT3 resident time at the gene loci
– Jonathan Martinez-Fabregas et al.
Cell Reports, 2020
MagReSyn® Ti-IMAC
The cGMP-Dependent Protein Kinase 2 Contributes to Cone Photoreceptor Degeneration in the Cnga3-Deficient Mouse Model of Achromatopsia
– Mirja Koch et al.
Int. J. Mol. Sci (2021)
MagReSyn® Ti-IMAC
An AKT2-specific nanobody that targets the hydrophobic motif induces cell cycle arrest, autophagy and loss of focal adhesions in MDA-MB-231 cells
– Tijs Merckaert et al.
Biomedicine & Pharmacotherapy (2020)
MagReSyn® Ti-IMAC
Zirconium(IV)-IMAC Revisited: Improved Performance and Phosphoproteome Coverage by Magnetic Microparticles for Phosphopeptide Affinity Enrichment
– et al.
J. Proteome Res. (2020)
MagReSyn® Zr-IMAC, Ti-IMAC, TiO2
An Unbiased Approach to Mapping the Signaling Network of the Pseudorabies Virus US3 Protein
– RJJ Jansens et al.
Pathogens (2020)
MagReSyn® Ti-IMAC
Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55
– Michael Plank et al.
Proteomics (2020)
MagReSyn® ZrO2, Ti-IMAC, TiO2
The Diabetes Gene JAZF1 Is Essential for the Homeostatic Control of Ribosome Biogenesis and Function in Metabolic Stress
– Ahmad Kobiita et al.
Cell Reports (2020)
MagReSyn® Ti-IMAC
Cannabinoid exposure in rat adolescence reprograms the initial behavioral, molecular, and epigenetic response to cocaine
– Maria Scherma et al.
PNAS (2020)
MagReSyn® Ti-IMAC
Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B
– Nadia Hégarat et al.
The EMBO Journal (2020)
MagReSyn® Ti-IMAC
Fluorescence fluctuation analysis reveals PpV dependent Cdc25 protein dynamics in living embryos
– Boyang Liu et al.
PLOS Genetics (2020)
MagReSyn® Ti-IMAC
HIPK4 is essential for murine spermiogenesis
– J. Aaron Crapster et al.
eLife (2020)
MagReSyn® Ti-IMAC, & TiO2
CYCA3;4 Is a Post-Prophase Target of the APC/CCCS52A2 E3 Ligase Controlling Formative Cell Divisions in Arabidopsis
– Alex Willems et al.
BioRxiv (2020)
MagReSyn® Ti-IMAC
Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition (DIA) without the need for spectral libraries
– Dorte B. Bekker-Jensen et al.
Nature Communications (2020)
MagReSyn® Ti-IMAC HP
Chemical genetics of AGC-kinases reveals shared targets of Ypk1, Protein Kinase A and Sch9
– Michael Plank et al.
Mol. Cell. Proteomics (2020)
MagReSyn® Ti-IMAC, ZrO2, & TiO2
A Compact Quadrupole-Orbitrap Mass Spectrometer with FAIMS Interface Improves Proteome Coverage in Short LC Gradients
– Dorte B. Bekker-Jensen et al.
Mol. Cell. Proteomics (2020)
MagReSyn® Ti-IMAC HP & Amine
Phosphorylation controls RNA binding and transcription by the influenza virus polymerase
– Anthony R Dawson et al.
BioRxiv (2020)
MagReSyn® Ti-IMAC
DYRK3-Controlled Phase Separation Organizes the Early Secretory Pathway
– Raffaella Gallo et al.
BioRxiv (2020)
MagReSyn® Ti-IMAC
R2‐P2 rapid‐robotic phosphoproteomics enables multidimensional cell signaling studies
– Mario Leutert et al.
Mol. Syst. Biol. (2019)
MagReSyn® Ti-IMAC, Zr-IMAC, & TiO2
Protein aggregation capture on Microparticles enables multi-purpose proteomics sample preparation
– Tanveer Singh Batth et al.
Mol. Cell. Proteomics (2019)
MagReSyn® HILIC, Ti-IMAC & TiO2
CD8 regulates insulin secretion and mediates postnatal and stress-induced expression of neuropeptides in pancreatic β cells
– Jing Xue et al.
Cell Reports 28 (2019)
MagReSyn® Ti-IMAC
Probability-based detection of phosphoproteomic uncertainty reveals rare signaling events driven by oncogenic kinase gene fusion
– Xavier Robin et al.
BioRxiv (2019)
MagReSyn® Ti-IMAC, MagReSyn® TiO2
Conduit integrity is compromised during acute lymph node expansion
– Victor G. Martinez et al.
BioRxiv (2019)
MagReSyn® Ti-IMAC
Proteasome-mediated remodeling of the proteome and phosphoproteome during kiwifruit pollen germination
– Candida Vannini et al.
Journal of Proteomics 192 (2019)
MagReSyn® Ti-IMAC
Direct Coupling of Dispersive Extractions with Magnetic Particles to Mass Spectrometry via Microfluidic Open Interface
– Marcos Gascon et al.
Analytical Chemistry (2019)
MagReSyn® Ti-IMAC
Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis
– Natalie M. Niemi et al.
Nature Communications (2019)
MagReSyn® Ti-IMAC
Early mannitol-triggered changes in the Arabidopsis leaf (phospho)proteome reveal growth regulators
– Natalia Nikonorova et al.
J. Experimental Botany 69 (2018)
MagReSyn® Ti-IMAC
Peanut Stunt Virus and Its Satellite RNA Trigger Changes in Phosphorylation in N. benthamiana Infected Plants at the Early Stage of the Infection
– Barbara Wrzesinkska et al.
Int. J of Moleecular Sciences 19 (2018)
MagReSyn® Ti-IMAC
Phosphoproteomic-based kinase profiling early in influenza virus infection identifies GRK2 as antiviral drug target
– Emilio Yángüez et al.
Nature Communications 9 (2018)
MagReSyn® Ti-IMAC
Ultra-high pressure (>30,000 psi) packing of capillary columns enhances depth of shotgun proteomic analyses
– Evgenia Shishkova et al.
Analytical Chemistry (2018)
MagReSyn® Ti-IMAC
The auxin-related CrRLK1L kinase ERULUS controls cell wall composition during root hair tip growth
– Sébastjen Schoenaers et al.
Current Biology 28 (2018)
MagReSyn® Ti-IMAC
Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphrases, using PRIMMUS
– Tony Ly et al.
ELIFE 6, pii: e27574 (2017)
MagReSyn® Ti-IMAC
HIV-1 activities T cell signaling independently of antigen to drive viral spread
– Alice C.L. Len et al.
Cell Reports 18 (2017)
MagReSyn® Ti-IMAC
Comparative genetic, proteomic and phosphoproteomic analysis of C. elegans embryos with a focus on ham-1/STOX and pig-1/MELK in dopaminergic neuron development
– Sarah-Lena Offenburger et al.
Nature Scientific Reports 7, 4314 (2017)
MagReSyn® Ti-IMAC
Cell-Specific Labeling for Analyzing Bidirectional Signaling by Mass Spectrometry
– Christopher J. Tape et al.
Kinase Signaling Networks pp 219-234. Part of the Methods in Molecular Biology, MIMB, volume 1636 (2017)
MagReSyn® Ti-IMAC, MagReSyn® TiO2
Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid Chromatography–Tandem Mass Spectrometry
– Mario Leutert et al.
Poly(ADP-Ribose) Polymerase: Methods and Protocols, Methods in Molecular Biology, vol. 1608 (2017)
MagReSyn® Ti-IMAC
GSK3α and GSK3β Phosphorylate Arc and Regulate its Degradation
– Agata Golds et al.
Frontiers in Molecular Neuroscience 10 (2017)
MagReSyn® Ti-IMAC
Proteome Profiling of Wheat Shoots from Different Cultivars
– Lam Dai Vu et al.
Frontiers in Plant Science (2017)
MagReSyn® Ti-IMAC
Phosphoproteomics with Activated Ion Electron Transfer Dissociation
– Nicholas M. Riley et al.
Analytical Chemistry (2017)
MagReSyn® Ti-IMAC
Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation
– Shin-ichiro Hiraga et al.
EMBO Reports (2017)
MagReSyn® Ti-IMAC
A Role for Barley Calcium-Dependent Protein Kinase CPK2a in the Response
– Agata Cieśla et al.
Frontiers in Plant Science 7 (2016), Article 1550
MagReSyn® Ti-IMAC
An up-to-date workflow for plant (phospho)proteomics identifies differential drought-responsive phosphorylation events in maize leaves
– Lam Dai Vu et al.
J. Proteome Research 15 (2016)
MagReSyn® Ti-IMAC
Oncogenic KRAS Regulates Tumor Cell Signaling via Stromal Reciprocation
– Christopher J. Tape et al.
Cell 165 (2016)
MagReSyn® Ti-IMAC, MagReSyn® TiO2
Cell-specific labelling enzymes for analysis of cell-cell communication in continuous co-culture
– Christopher J. Tape et al.
Mol. Cell. Proteomics 13 (2014)
MagReSyn® Ti-IMAC
Reproducible automated phosphopetide enrichment using magnetic TiO2 and Ti-IMAC
– Christopher J. Tape et al.
Analytical Chemistry 86 (2014)
MagReSyn® Ti-IMAC, MagReSyn® TiO2
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