Multi-mode HILIC microparticles for sample clean-up pre-Mass Spectrometry

The range of possible sample contaminants, variability in starting material, & range of possible reagents for sample preparation has resulted in variability in data generated from mass spectrometry. Current methodologies and tools for sample clean-up prior to digestion and MS analysis require the use of in-line column chromatography, desalting columns/membranes, and/or packed tips for partitioning. Although some of these techniques are broadly applicable, they are not routinely automatable, or don’t provide the option for parallel processing required for high-throughput. In order to fully automate clean-up we have developed a versatile magnetic mixed-mode HILIC with broad applicability for the removal of common contaminants required in the solubilization and denaturation of proteins. The automation of sample processing using magnetic beads reduces sample handling, and providing massively parallel sample processing, and improves reproducibility.

MagReSyn® HILIC provides a highly reproducible and fully automatable solution for parallel protein sample clean-up prior to MS analysis. The product is compatible with a range of common contaminants which interfere in the generation of high quality data from MS analysis of samples. 

Please review our extensive range of application notes and posters to see how MagReSyn® HILIC can be used for your sample preparation. 

Support: Proprietary polymer microspheres containing iron oxide (magnetite)
Bead size: ~5-10 µm
Formulation: 20 suspension in 20% ethanol


Product Resources

Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.

Citations and References

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–  Emeka John Dingwoke et al.

In Review: Scientific Reports (2021)


SWATH-MS based proteomic profiling of pancreatic ductal adenocarcinoma tumours reveals the interplay between the extracellular matrix and related intracellular pathways

–  Ekene Emmanuel Nweke et al.

PLOS ONE (2020)


KLF4 Recruits SWI/SNF to Increase Chromatin Accessibility and Reprogram
the Endothelial Enhancer Landscape under Laminar Shear Stress

–  Jan-Renier A.J. Moonen et al.

BioRxiv (2020)


Inflammatory and antimicrobial properties differ between vaginal Lactobacillus isolates from South African women with non-optimal versus optimal microbiota

–  Monalisa T. Manhanzva et al.

Nature Scientific Reports (2020)


Open search unveils modification patterns in formalin-fixed, paraffin-embedded thermo HCD and SCIEX TripleTOF shotgun proteomes

–  David L. Tabb et al.

Int J of Mass Spectrometry (2020)


Microbial function and genital inflammation in young South African women at high risk of HIV infection

–   Arghavan Alisoltani et al.

BioRxiv (2020)


Protein aggregation capture on microparticles enables multi-purpose proteomics sample preparation

–   Tanveer Singh Batth et al.

Mol. Cell. Proteomics (2019)

MagReSyn® NTA, TiO2, & HILIC

The study of degradation mechanisms of glyco-engineered plant produced anti-rabies monoclonal antibodies E559 and 62-71-3

–   Sindiswe Buthelezi et al.

PLOS ONE (2018)