MagReSyn® TiO2

TiO– functional microparticles for highly-specific phosphopeptide enrichment

Protein phosphorylation plays a pivotal role in most cellular processes, with 30% of the proteome transiently phosphorylated. It is therefore a widely studied post-translational modification. However, the low-abundant nature of phosphopeptides, the low stoichiometry of the modification, and the physico-chemical properties of phosphorylated peptides make proteome-wide characterization of phosphorylation a significant challenge to proteomics researchers.

Consequently, technologies than can specifically enrich phosphopeptides, and are compatible with mass spectrometric analyses, are highly desirable. MagReSyn® TiO2 microparticles allow highly specific, reproducible enrichment of phosphopeptides from complex biological samples such as protein digests. Titanium dioxide enrichment shows selective affinity for phosphoserine (pSer), phosphothreonine (pThr) and phosphotyrosine (pTyr) residues. MagReSyn® TiO2 microparticles have been engineered to achieve the ultimate specificity, outperforming competitor products, with excellent phosphopeptide recovery. 

MagReSyn® TiO2 microparticles may be used alone or in combination with MagReSyn® ZrO2, MagReSyn® Zr-IMAC and/or MagReSyn® Ti-IMAC microparticles to enrich diverse types of phosphopeptides for comprehensive phosphoproteomics analyses. Products for phosphopeptide enrichment are validated for the intended application using our stringent QC procedures. 

IMPORTANT NOTE: To ensure optimal enrichment using this product please ensure your sample is free of contaminating compounds by using sample preparation procedures such as PAC, and ensure sufficient mixing to ensure beads remain in suspension for good contact between your sample and the magnetic beads. Please read the instruction guide carefully before using the product, and ensure you centrifuge samples prior to LC MS analysis to remove possible insoluble contaminants.

Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Bead size: ~5-10 µm
Formulation: 25 mg.ml-1 suspension in 20% ethanol

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Product Resources

MagReSyn® TiO2 Performance Superiority

Comparative assessment of MagReSyn® TiO2 (green bars) vs a leading competitor (orange bars) for phosphopeptide enrichment.
32P labelled CHK1
tryptic digests were enriched for phosphopeptides and analyzed by LC/MS/MS.
MagReSyn® TiO2 
showed exceptional specificity with up to 100% of identified peptides being phosphorylated, vs 78% for the competitor.
Phosphopeptide recovery was
78% using MagReSyn® TiO2 and 70% using alternate TiO2.

 

Citation and Reference Examples

Please review our comprehensive method library to find the most relevant reference material for your particular application.

Heterocellular OSM-OSMR signalling reprograms fibroblasts to promote pancreatic cancer growth and metastasis

–  Brian Y. Lee et al.

Nature Communications (2021)

MagReSyn® TiO2

Zirconium(IV)-IMAC Revisited: Improved Performance and Phosphoproteome Coverage by Magnetic Microparticles for Phosphopeptide Affinity Enrichment

–  Ignacio Arribas Diez et al.

J. Proteome Res. (2020)

MagReSyn® Zr-IMAC, Ti-IMAC, TiO2

Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc55

–  Michael Plank et al.

Proteomics (2020)

MagReSyn® Ti-IMAC, TiO2, & ZrO2

Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in protein translation

–  M. Regina Scarpin et al.

BioRxiv (2020)

MagReSyn® TiO2

HIPK4 is essential for murine spermiogenesis

–   J. Aaron Crapster et al.

eLife (2020)

MagReSyn® Ti-IMAC & TiO2

Chemical Genetics of AGC-kinases Reveals Shared Targets of Ypk1, Protein Kinase A and Sch9

–   Michael Plank et al.

Mol. Cell. Proteomics (2020)

MagReSyn® Ti-IMAC, ZrO2, & TiO2

TERMS AND CONDITIONS
Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.