For rapid, automated chymotryptic digestion of proteins for mass spectrometry sample preparation
MagReSyn® Chymotrypsin microparticles consist of covalently immobilized sequencing grade chymotrypsin (TLCK treated to prevent tryptic activity) for protein digestion prior to mass spectrometry analysis. The porous high capacity MagReSyn® microparticle technology allows for high volumetric enzyme activity allowing for rapid digestion. The magnetically immobilized chymotrypsin enables automated and miniaturized digestion workflows improving reproducibility. The enzyme cleaves at bulky hydrophobic residues thus providing complementary protein coverage as compared to magnetically immobilized trypsin digestion (see MagReSyn® Trypsin).
Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Digestion ratio: 20 µl (200 µg) microparticles per 50 µg protein sample
Bead size: ~5-10 µm
Formulation: 10 mg.ml-1 suspension in 50 mM acetic acid
MagReSyn® Chymotrypsin Performance Superiority
- High Chymotrypsin content ensures rapid digestion
Improved stability and compatibility
- Multipoint covalent attachment of chymotrypsin
- Low chymotrypsin leaching reduces sample contamination
- Increased sequence coverage – complementary to chymotrypsin cleavage
- Suitable for automated digestion on magnetic handling stations
- Reduce sample preparation variability and improves reproducibility
Digestion with MagReSyn® Chymotrypsin provides good protein coverage for a range of proteins,
including large and difficult to digest examples such as Thyroglobulin and Myoglobin.
TERMS AND CONDITIONS
Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.