Method Library
This resource aims to make methods more accessible to researchers, and help identify the most relevant workflows for your current application. We would appreciate your participation in keeping this database up to date, so please report any errors such as broken links, missing information, or publications that are not included to info@resynbio.com.
Resource Title | Year | Resource | Application | Product | Sample Source | Strategy | Extraction | Clean-up | Digestion | Quantification |
---|---|---|---|---|---|---|---|---|---|---|
Preparation and HILIC SPE clean-up of SDS extracted plasma proteins for MS analysis | 2022 | Method | Digest preparation | HILIC | Biofluids | Manual | Detergent | Hydrophilic affinity | LysC, Trp | Label-free |
Preparation and HILIC SPE clean-up of urinary proteins for MS analysis | 2022 | Method | Digest preparation | HILIC | Biofluids | Manual | Detergent | Hydrophilic affinity | LysC, Trp | Label-free |
Tissue-wide Effects Overrule Cell-intrinsic Gene Function in Cortical Projection Neuron Migration | 2022 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian tissue | Manual | Kit | Kit | Trp | Isobaric-tagging |
ULK4 and Fused/STK36 interact to mediate assembly of a motile flagellum | 2022 | Publication | BioID | Streptavidin | Mosquito cells | Manual | Detergent | Affinity capture | N/A | Label-free |
Assessment of Streptavidin Bead Binding Capacity to Improve Quality of Streptavidin-based Enrichment Studies | 2021 | Publication | Affinity capture | Streptavidin | N/A | N/A | N/A | N/A | N/A | Label-free |
Liver-fibrosis-activated transcriptional networks govern hepatocyte reprogramming and intra-hepatic communication | 2021 | Publication | Digest preparation | HILIC | Mammalian tissue | Manual | Chaotrope | Precipitation on-bead | LysC, Trp | Label-free |
Bromodomain factor 5 is an essential transcriptional regulator of the Leishmania genome | 2021 | Publication | BioID | Streptavidin | Mammalian cells, Protozoa | Manual | Detergent | Affinity capture | Trp | Label-free |
Dissecting the Functional Selectivity of TGF? Signaling Pathway Components Using Genome A | 2021 | Publication | Digest preparation, Phosphopeptide enrichment | Amine, Ti-IMAC | Mammalian tissue | Automated | Detergent | Precipitation on-bead | LysC, Trp | Isobaric-tagging |
Loquacious Modulates Flaviviral RNA Replication in Mosquito Cells | 2021 | Publication | Affinity capture | Streptavidin | Insect cells | Manual | Detergent | Affinity capture | N/A | Label-free |
Dysregulated healing responses in diabetic wounds occur in the early stages postinjury | 2021 | Publication | Digest preparation | HILIC | Mammalian tissue | Manual | Organic | Hydrophilic affinity | Trp | Isobaric-tagging |
The cGMP-Dependent Protein Kinase 2 Contributes to Cone Photoreceptor Degeneration in the Cnga3-Deficient Mouse Model of Achromatopsia | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian tissue | Manual | N/A | Precipitation in-solution | Trp | Label-free |
Taylor-made production of pyrimidine nucleoside-5′-monophosphate analogues by highly stabilized mutant uracil phosphoribosyltransferase from Toxoplasma gondii | 2021 | Publication | Immobilization | Amine, Ni-NTA | Enzyme | N/A | N/A | N/A | N/A | N/A |
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases | 2021 | Publication | Immobilization | Amine | Enzyme | N/A | N/A | N/A | N/A | N/A |
Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution | 2021 | Publication | Digest preparation, Phosphopeptide enrichment | Amine, Ti-IMAC | Mammalian tissue | Automated | Native, Detergent | Precipitation on-bead | LysC, Trp | Label-free |
CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Detergent | Precipitation in-solution | Trp | Metabolic labelling |
Identifying cytokine signaling signatures in primary human Th-1 cells by phospho-proteomics analysis | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Detergent | Precipitation in-solution | Trp | Metabolic labelling |
Molecular roadmap of the healthy stress response in the mouse hippocampus | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian tissue | Manual | Detergent | Filter-based | Trp | Label-free |
HNF4A is required to specify glucocorticoid action in the liver | 2021 | Publication | ChIP | Protein G | Mammalian tissue | Manual | N/A | N/A | N/A | N/A |
Rapid auxin-mediated phosphorylation of Myosin regulates trafficking and polarity in Arabidopsis | 2021 | Publication | Phosphopeptide enrichment | TiO2, Ti-IMAC | Plant | Manual | Detergent | Hybrid | Trp | Label-free |
Venom Proteomic Analysis of Two Medically Important Nigerian Viper (Echis Ocellatus and Bitis Arietans) Snake Species | 2021 | Publication | Digest preparation | HILIC | Snake venom | Manual | N/A | Hybrid | Trp | Label-free |
Monocyte Released HERV-K dUTPase Engages TLR4 and MCAM Causing Endothelial Mesenchymal Transition | 2021 | Publication | Peptide clean-up | HILIC | Mammalian cells | Manual | Native | Hydrophilic affinity | LysC, Trp | Label-free |
Proteomics of resistance to Notch1 inhibition in acute lymphoblastic leukemia reveals targetable kinase signatures | 2021 | Publication | Digest preparation, Phosphopeptide enrichment | Amine, Ti-IMAC | Mammalian cells | Automated | Detergent | Precipitation on-bead | LysC, Trp | Isobaric-tagging, Label-free |
KF Method: Automated Phosphopeptide Enrichment using MagReSyn® IMAC | 2021 | Method | Phosphopeptide enrichment | Ti-IMAC, Zr-IMAC | N/A | Automated | N/A | N/A | N/A | Label-free |
High-throughput phosphoproteomics profiling of non-small cell lung cancer tissues | 2021 | Poster | Phosphopeptide enrichment | Ti-IMAC | Mammalian tissue | Automated | N/A | In-solution | Trp | Label-free |
The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted antiviral signaling and host translation | 2021 | Publication | BioID | Streptavidin | Mammalian cells | Automated | Detergent | Affinity capture | LysC, Trp | Label-free |
Product Guide MagReSyn® Trypsin | 2021 | Method | Digest preparation | Trypsin | N/A | Manual | N/A | In-solution | Trp | N/A |
Product Guide MagReSyn® Chymotrypsin | 2021 | Method | Digest preparation | Chymotrypsin | Recombinant protein | Manual | N/A | In-solution | ChymoTrp | N/A |
Competitive binding of STATs to receptor phospho-Tyr motifs accounts for altered cytokine responses | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Detergent | Precipitation in-solution | Trp | Metabolic labelling |
Drug-bound and -free outward-facing structures of a multidrug ABC exporter point to a swing mechanism | 2021 | Publication | HDX | Zr-IMAC | Recombinant protein | N/A | N/A | N/A | Pepsin | HDX |
Antagonistic cell surface and intracellular auxin signalling regulate plasma membrane H+ fluxes for root growth | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Manual | Detergent | Filter-based | Trp | Label-free |
The membrane-localized protein kinase MAP4K4/TOT3 regulates thermomorphogenesis | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Manual | Native | Precipitation in-solution | LysC, Trp | Label-free |
The cGMP-Dependent Protein Kinase 2 Contributes to Cone Photoreceptor Degeneration in the Cnga3-Deficient Mouse Model of Achromatopsia | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian tissue | Manual | N/A | Precipitation in-solution | Trp | Label-free |
Unraveling the MAX2 Protein Network in Arabidopsis thaliana: Identification of the Protein Phosphatase PAPP5 as a Novel MAX2 Interactor | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Manual | Native | Affinity capture | LysC, Trp | Label-free |
An AKT2-specific nanobody that targets the hydrophobic motif induces cell cycle arrest, autophagy and loss of focal adhesions in MDA-MB-231 cells | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Chaotrope | In-solution | LysC, Trp | Label-free |
Evaluation of Protein Purification Techniques and Effects of Storage Duration on LC-MS/MS Analysis of Archived FFPE Human CRC Tissues | 2021 | Publication | Digest preparation | HILIC | FFPE | Automated | Detergent | Hydrophilic affinity | Trp | Label-free |
Functional studies of CpSRP54 in diatoms show that themechanism of thylakoid protein insertion differs from that inplants and green algae | 2021 | Publication | Digest preparation | HILIC | Mammalian cells | Automated | Detergent | Hydrophilic affinity | Trp | Label-free |
Proteomic analysis of three medically important Nigerian Naja (Naja haje, Naja katiensis and Naja nigricollis) snake venoms | 2021 | Publication | Digest preparation | HILIC | Snake venom | Manual | N/A | Hydrophilic affinity | Trp | Label-free |
Plasmodium falciparum Calcium Dependent Protein Kinase 4 is critical for male gametogenesis and transmission to the mosquito vector | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC, Zr-IMAC | Plasmodium | Automated | Detergent | Precipitation in-solution | Trp | Label-free |
Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion | 2021 | Publication | Digest preparation, Phosphopeptide enrichment | HILIC, Ti-IMAC | Mammalian cells, Other | Manual | Detergent | Precipitation on-filter, Affinity capture | Trp | Isobaric-tagging, Label-free |
DYRK3-Controlled Phase Separation Organizes the Early Secretory Pathway | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Automated | N/A | Other | Trp | Label-free |
Phosphorylation controls RNA binding and transcription by the influenza virus polymerase | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Chaotrope | Precipitation in-solution | Trp | Label-free |
Modulation of immune cell reactivity with cis-binding Siglec agonists | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Detergent | Precipitation on-filter | Trp | Isobaric-tagging |
A Compact Quadrupole-Orbitrap Mass Spectrometer with FAIMS Interface Improves Proteome Coverage in Short LC Gradients | 2020 | Publication | Digest preparation, Phosphopeptide enrichment | Amine, Ti-IMAC | Mammalian cells, Mammalian tissue | Automated | Detergent | Precipitation on-bead | LysC, Trp | Label-free |
Chemical Genetics of AGC-kinases Reveals Shared Targets of Ypk1, Protein Kinase A and Sch9 | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC, TiO2, ZrO2 | Yeast cells | Manual | N/A | Precipitation in-solution | Trp | Label-free |
Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | Yeast cells | Manual | Detergent | Precipitation in-solution | LysC, Trp | Label-free |
Functional studies of CpSRP54 in diatoms show that the mechanism of thylakoid protein insertion differs from that in plants and green algae | 2020 | Publication | Digest preparation | HILIC | Algae | Automated | Detergent | Hydrophilic affinity | Trp | Label-free |
SWATH-MS based proteomic profiling of pancreatic ductal adenocarcinoma tumours reveals the interplay between the extracellular matrix and related intracellular pathways | 2020 | Publication | Digest preparation | HILIC | Mammalian tissue | Automated | Detergent | Hydrophilic affinity | Trp | Label-free |
CDK8 fine-tunes IL-6 transcriptional activities by limiting STAT3 resident time at the gene loci | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Manual | Native | Precipitation in-solution | LysC, Trp | Label-free |
The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Manual | Native | Precipitation in-solution | LysC, Trp | Label-free |
Alterations in the CO2 availability induce alterations in the phosphoproteome of the cyanobacterium Synechocystis sp. PCC 6803 | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC, TiO2 | Bacterial cells | Manual | Detergent | Precipitation in-solution | LysC, Trp | Label-free |
CYCA3;4 Is a Post-Prophase Target of the APC/CCCS52A2 E3-Ligase Controlling Formative Cell Divisions in Arabidopsis | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Detergent | N/A | Trp | Metabolic labelling |
HIPK4 is essential for murine spermiogenesis | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC, TiO2 | Mammalian cells | Manual | Detergent | Precipitation in-solution | LysC, Trp | Isobaric-tagging |
Fluorescence fluctuation analysis reveals PpV dependent Cdc25 protein dynamics in living embryos | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | Drosophila | Manual | Native | Gel-based | Trp (in-gel) | Label-free |
Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Automated | Detergent | Precipitation in-solution | LysC, Trp | Isobaric-tagging |
Cannabinoid exposure in rat adolescence reprograms the initial behavioral, molecular, and epigenetic response to cocaine | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC, Zr-IMAC | Mammalian tissue | Manual | Chaotrope | Precipitation in-solution | LysC, Trp | Isobaric-tagging |
The Diabetes Gene JAZF1 Is Essential for the Homeostatic Control of Ribosome Biogenesis and Function in Metabolic Stress | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Detergent | Filter-based | N/A | Label-free |
Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC, TiO2, ZrO2 | Yeast cells | Manual | Chaotrope | In-solution | LysC, Trp | Metabolic labelling |
An Unbiased Approach to Mapping the Signaling Network of the Pseudorabies Virus US3 Protein | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Chaotrope | In-solution | LysC, Trp | Label-free |
Zirconium(IV)-IMAC Revisited: Improved Performance and Phosphoproteome Coverage by Magnetic Microparticles for Phosphopeptide Affinity Enrichment | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC, Zr-IMAC, TiO2 | Mammalian cells | Both | Detergent | Precipitation in-solution | Trp | Label-free |
Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in translation | 2020 | Publication | Phosphopeptide enrichment | TiO2 | Plant | Manual | Native | In-solution | Trp | Isobaric-tagging |
Magnetic micro-macro biocatalysts applied to industrial bioprocesses | 2020 | Publication | Immobilization | Ni-NTA | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
Magnetic micro-macro biocatalysts applied to industrial bioprocesses | 2020 | Publication | Immobilization | Amine | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
Novel kinase platform for the validation of the anti-tubercular activities of Pelargonium sidoides (Geraniaceae) | 2020 | Publication | Protein Purification | Ni-NTA | Bacterial cells | Manual | Native | N/A | N/A | N/A |
Product Guide MagReSyn® TiO2 | 2020 | Method | Phosphopeptide enrichment | TiO2 | N/A | Manual | N/A | N/A | N/A | Label-free |
Evaluation of Novel Supports for Selective and Efficient Enrichment of Phosphorylated Peptides | 2020 | Method | Phosphopeptide enrichment | TiO2, ZrO2 | N/A | Automated | N/A | N/A | N/A | Label-free |
Product Guide MagReSyn® ZrO2 | 2020 | Method | Phosphopeptide enrichment | ZrO2 | N/A | Manual | N/A | N/A | N/A | Label-free |
Product Guide MagReSyn® Ti-IMAC HP | 2020 | Method | Phosphopeptide enrichment | Ti-IMAC | N/A | Manual | N/A | N/A | N/A | Label-free |
Product Guide MagReSyn® Ti-IMAC | 2020 | Method | Phosphopeptide enrichment | Ti-IMAC | N/A | Manual | N/A | N/A | N/A | Label-free |
A Data Independent Acquisition Workflow Enables the Profiling of Thousands of Human Cancer Tissues for Precision Oncology | 2020 | Poster | Phosphopeptide enrichment | Ti-IMAC | Mammalian tissue | Automated | Unknown | In-solution | Trp | Label-free |
Fast and reproducible phosphoproteomics using MagReSyn® Amine and Ti-IMAC HP magnetic beads and the Evosep One | 2020 | Application note | Digest preparation, Phosphopeptide enrichment | Amine, Ti-IMAC | Mammalian cells | Automated | Detergent | Precipitation on-bead | LysC, Trp | Label-free |
High Throughput Proteome and Phosphoproteome Sample Processing Couple to Fast Gradient DIA | 2020 | Poster | Phosphopeptide enrichment | Ti-IMAC | Mammalian tissue | Automated | Detergent | Precipitation on-bead | LysC, Trp | Label-free |
High Throughput Proteome and Phosphoproteome Sample Processing Couple to Fast Gradient DIA | 2020 | Poster | Digest preparation | Amine | Mammalian tissue | Automated | Detergent | Precipitation on-bead | LysC, Trp | Label-free |
Product Guide MagReSyn® Zr-IMAC HP | 2020 | Method | Phosphopeptide enrichment | Zr-IMAC | N/A | Manual | N/A | N/A | N/A | Label-free |
Product Guide MagReSyn® Zr-IMAC | 2020 | Method | Phosphopeptide enrichment | Zr-IMAC | N/A | Manual | N/A | N/A | N/A | Label-free |
Zirconium(IV) IMAC-based enrichment for mass spectrometry driven phosphoproteomics | 2020 | Poster | Phosphopeptide enrichment | Ti-IMAC, Zr-IMAC | Mammalian cells | Automated | N/A | N/A | N/A | Label-free |
KF Method: Automated clean-up and on-bead digestion with MagReSyn® HILIC | 2020 | Method | Digest preparation | HILIC | N/A | Automated | Detergent | Hydrophilic affinity | Trp | Label-free |
Sustainable synthesis of uridine-5?-monophosphate analogues by immobilized uracil phosphoribosyltransferase from Thermus thermophilus | 2020 | Publication | Immobilization | Amine | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
Identification of MAMP-Responsive Plasma Membrane-Associated Proteins in Arabidopsis thaliana Following Challenge with Different LPS Chemotypes from Xanthomonas campestris | 2020 | Publication | Affinity capture | Streptavidin | Bacterial cells | Manual | N/A | N/A | N/A | N/A |
Biochemical Pulldown of mRNAs and Long Noncoding RNAs from Cellular Lysates Coupled with Mass Spectrometry to Identify Protein Binding Partners | 2020 | Publication | Affinity capture | Streptavidin | Mammalian cells | Manual | Detergent | Gel-based | Trp | Label-free |
Multiplexed Proximity Biotinylation Coupled to Mass Spectrometry for Defining Integrin Adhesion Complexes | 2020 | Publication | BioID | Streptavidin | Mammalian cells | Manual | Detergent | Gel-based | Trp | Label-free |
Topological features of integrin adhesion complexes revealed by multiplexed proximity biotinylation | 2020 | Publication | BioID | Streptavidin | Mammalian cells | Manual | Detergent | Gel-based | Trp | Label-free |
BioID based proteomic analysis of the Bid interactome identifies novel proteins involved in cell cycle dependent apoptotic priming | 2020 | Publication | BioID | Streptavidin | Mammalian cells | Manual | Detergent | Gel-based | Trp | Label-free |
Plant-based production of highly potent anti-HIV antibodies with engineered posttranslational modifications | 2020 | Publication | Affinity capture | Protein A | Plant | Manual | Native | N/A | N/A | N/A |
Hypoxia regulates GR function through multiple mechanisms involving microRNAs 103 and 107 | 2020 | Publication | ChIP | Protein G | Mammalian cells | Manual | N/A | N/A | N/A | qPCR |
Insights from the First Phosphopeptide Challenge of the MS Resource Pillar of the HUPO Human Proteome Project | 2020 | Publication | Phosphopeptide enrichment | Ti-IMAC | HUPO Synthetic | Both | N/A | N/A | N/A | N/A |
Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis | 2019 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian tissue | Manual | Chaotrope | Precipitation in-solution | Trp double digest | Isobaric-tagging |
Proteasome-mediated remodeling of the proteome and phosphoproteome during kiwifruit pollen germination | 2019 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Manual | N/A | Hybrid | N/A | Label-free |
Conduit integrity is compromised during acute lymph node expansion | 2019 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Automated | Chaotrope | Filter-based | N/A | Isobaric-tagging |
Probability-based detection of phosphoproteomic uncertainty reveals rare signaling events driven by oncogenic kinase gene fusion | 2019 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Detergent | Precipitation in-solution | Trp | Label-free |
CDK8 Regulates Insulin Secretion and Mediates Postnatal and Stress-Induced Expression of Neuropeptides in Pancreatic ? Cells | 2019 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Detergent | Filter-based | N/A | Label-free |
Protein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation | 2019 | Publication | Digest preparation, Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Detergent | Precipitation on-bead | LysC, Trp | Label-free |
R2-P2 rapid-robotic phosphoproteomics enables multidimensional cell signaling studies | 2019 | Publication | Phosphopeptide enrichment | Ti-IMAC, Zr-IMAC, TiO2 | Yeast cells | Automated | Chaotrope | Precipitation on-bead | LysC, Trp | Label-free |
Development of fully automated pipeline for phosphoproteome profiling optimisation of phosphopeptide enrichment | 2019 | Poster | Phosphopeptide enrichment | Ti-IMAC, Zr-IMAC, TiO2, ZrO2, Amine, HILIC, SAX | Recombinant protein, Mammalian cells | Automated | Detergent, Chaotrope | In-solution, Precipitation on-bead, Hydrophilic affinity, Ion exchange | Trp | Isotope-labelled peptides, Label-free |
Development of a fully automated high throughput magnetic workflow for phosphoproteome profiling | 2019 | Poster | Phosphopeptide enrichment | Ti-IMAC, Zr-IMAC, TiO2 | Mammalian cells | Automated | Detergent | Precipitation on-bead | Trp | Label-free |
Development of fully automated pipeline for phosphoproteome profiling | 2019 | Poster | Digest preparation, Phosphopeptide enrichment | Ti-IMAC, SAX, HILIC | Mammalian cells | Automated | Detergent, Chaotrope | Precipitation on-bead, Hydrophilic affinity, Ion exchange | Trp | Label-free |
PEPCLU MagReSyn HILIC Clean-Up of Peptides Post Protein Digestion | 2019 | Method | Peptide clean-up | HILIC | N/A | Manual | Detergent | Hydrophilic affinity | N/A | Label-free |
RAPOBD HILIC Clean-Up of Reduced & Alkylated Proteins with on Bead Digestion | 2019 | Method | Digest preparation | HILIC | Mammalian cells | Manual | Detergent | Hydrophilic affinity | Trp | Label-free |
POBRAD HILIC Clean-Up of proteins with on-bead reduction, alkylation and digestion | 2019 | Method | Digest preparation | HILIC | Mammalian cells | Manual | Detergent | Hydrophilic affinity | Trp | Label-free |
Improved reproducibility of automated sample preparation using HILIC magnetic beads automated by Hamilton Robotics | 2019 | Application note | Digest preparation | HILIC | N/A | Automated | Detergent | Hydrophilic affinity | Trp | Label-free |
Development of a fully automated magnetic workflow for phosphoproteome profiling | 2019 | Poster | Digest preparation, Phosphopeptide enrichment | Ti-IMAC, Zr-IMAC, TiO2, ZrO2, SAX, HILIC | Mammalian cells | Automated | Detergent, Chaotrope | Hydrophilic affinity, Ion exchange | Trp | Label-free |
Product Guide MagResyn® Amine - activation with epoxide for protein immobilization | 2019 | Method | Immobilization | Amine | N/A | Manual | N/A | N/A | N/A | N/A |
Product Guide MagReSyn® Streptavidin | 2019 | Method | Affinity capture | Streptavidin | N/A | Manual | N/A | N/A | N/A | N/A |
Product Guide MagReSyn® Streptavidin MAX | 2019 | Method | Affinity capture | Streptavidin | N/A | Manual | N/A | N/A | N/A | N/A |
Product Guide MagReSyn® Protein A | 2019 | Method | Affinity capture | Protein A | N/A | Manual | N/A | N/A | N/A | N/A |
Product Guide MagReSyn® Protein A MAX | 2019 | Method | Affinity capture | Protein A | N/A | Manual | N/A | N/A | N/A | N/A |
Product Guide MagReSyn® Protein G | 2019 | Method | Affinity capture | Protein G | N/A | Manual | N/A | N/A | N/A | N/A |
p38α MAPK proximity assay reveals a regulatory mechanism of alternative splicing in cardiomyocytes | 2019 | Publication | Affinity capture | Protein G | Mammalian tissue | Manual | Detergent | Gel-based | N/A | N/A |
An improved method for quantitative ChIP studies of nuclear receptor function | 2019 | Publication | ChIP | Protein G | Mammalian tissue | Manual | N/A | In-solution | N/A | qPCR |
The Auxin-Regulated CrRLK1L Kinase ERULUS Controls Cell Wall Composition during Root Hair Tip Growth | 2018 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Manual | Native | Precipitation in-solution | LysC, Trp | Label-free |
Ultra-High Pressure (>30,000 psi) Packing of Capillary Columns Enhancing Depth of Shotgun Proteomic Analyses | 2018 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells, Mammalian tissue | Manual | Chaotrope | Precipitation in-solution | LysC, Trp | Isobaric-tagging |
Magnetic HILIC: An enabling & versatile tool for robust automated MS sample preparation workflows | 2018 | Poster | Digest preparation | HILIC | Mammalian cells | Automated | Detergent | Hydrophilic affinity | Trp | Label-free |
Magnetic HILIC: An enabling & versatile tool for robust automated MS sample preparation workflows | 2018 | Poster | Peptide clean-up | HILIC | Mammalian cells | Automated | Detergent | Hydrophilic affinity | Trp | Label-free |
Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives | 2018 | Publication | Immobilization | Amine | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
Enzymatic Synthesis of Therapeutic Nucleosides using a Highly Versatile Purine Nucleoside 2-DeoxyribosylTransferase from Trypanosoma brucei | 2018 | Publication | Immobilization | Amine | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
One-Pot, One-Step Production of Dietary Nucleotides by Magnetic Biocatalysts | 2018 | Publication | Immobilization | Amine | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
Immune genes are primed for robust transcription by proximal long noncoding RNAs located in nuclear compartments | 2018 | Publication | Affinity capture | Streptavidin, Protein A, Protein G | Mammalian cells | Manual | Detergent | Gel-based | N/A | qPCR |
REVERBa couples the circadian clock to hepatic glucocorticoid action | 2018 | Publication | ChIP, Affinity capture | Protein A, Protein G | Mammalian cells | Manual | N/A | N/A | N/A | qPCR |
LRP1 is required for novobiocin-mediated fibronectin turnover | 2018 | Publication | Affinity capture | Protein A | Mammalian cells | Manual | Detergent | Gel-based | Trp | N/A |
Circadian clock component REV-ERB? controls homeostatic regulation of pulmonary inflammation | 2018 | Publication | Affinity capture | Protein A | Mammalian cells | Manual | Detergent | Gel-based | Trp | N/A |
Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation | 2017 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Automated | Detergent | Precipitation in-solution | Trp, AspN | Isobaric-tagging |
Phosphoproteomics with Activated Ion Electron Transfer Dissociation | 2017 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian tissue | Manual | Chaotrope | Precipitation in-solution | Trp | Label-free |
Proteome Profiling of Wheat Shoots from Different Cultivars | 2017 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Manual | Native | Precipitation in-solution | LysC, Trp | Label-free |
GSK3α and GSK3β Phosphorylate Arc and Regulate its Degradation | 2017 | Publication | Phosphopeptide enrichment | Ti-IMAC | Recombinant protein | Manual | Detergent | Other | Trp, Pepsin | Label-free |
Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid ChromatographyTandem Mass Spectrometry | 2017 | Publication | ADP-ribosylation enrichment | Ti-IMAC | Recombinant protein | Manual | Chaotrope | Filter-based | Trp | Label-free |
Cell-Specific Labeling for Analyzing Bidirectional Signaling by Mass Spectrometry | 2017 | Publication | Phosphopeptide enrichment | Ti-IMAC, TiO2 | Mammalian cells | Manual | Chaotrope | Filter-based | Trp | Metabolic labelling |
Comparative genetic, proteomic and phosphoproteomic analysis of C. elegans embryos with a focus on ham-1/STOX and pig-1/MELK in dopaminergic neuron development | 2017 | Publication | Phosphopeptide enrichment | Ti-IMAC | C. elegans | Automated | Chaotrope | In-solution | LysC, Trp | Label-free |
HIV-1 Activates T Cell Signaling Independently of Antigen to Drive Viral Spread | 2017 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Detergent | Filter-based | Trp double digest | Metabolic labelling |
Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS | 2017 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Detergent | Precipitation on-bead | LysC, Trp | Isobaric-tagging |
Reactivation of MagReSyn® Ti-IMAC | 2017 | Method | Phosphopeptide enrichment | Ti-IMAC | N/A | N/A | N/A | N/A | N/A | N/A |
Product Guide MagReSyn® HILIC | 2017 | Method | Digest preparation | HILIC | N/A | Manual | Detergent | Hydrophilic affinity | Trp | Label-free |
Universal Unbiased pre-MS Clean-Up Using Magnetic HILIC Microparticles for SPE | 2017 | Poster | Digest preparation | HILIC | Mammalian cells | Automated | Detergent | Hydrophilic affinity | Trp | Label-free |
Dealing with the Dirty Dozen: Universal Unbiased preMS Clean-Up Using Magnetic HILIC SPE | 2017 | Poster | Digest preparation | HILIC | Mammalian cells | Automated | Detergent | Hydrophilic affinity | Trp | Label-free |
Product Guide MagReSyn® Amine | 2017 | Method | Immobilization | Amine | N/A | Manual | N/A | N/A | N/A | N/A |
Structural and functional characterization of thermostable biocatalysts for the synthesis of 6-aminopurine nucleoside-5?-monophospate analogues | 2017 | Publication | Immobilization | Amine | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
Product Guide MagReSyn® Carboxyl | 2017 | Method | Immobilization | Carboxyl | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
Identification of lipopolysaccharide-interacting plasma membrane-type proteins in Arabidopsis thaliana | 2017 | Publication | Affinity capture | Streptavidin | Plant | Manual | Native | Gel-based | Trp | N/A |
Up-to-Date Workflow for Plant (Phospho)proteomics Identifies Differential Drought-Responsive Phosphorylation Events in Maize Leaves | 2016 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Manual | Native | Precipitation in-solution | LysC, Trp | Label-free |
Oncogenic KRAS Regulates Tumor Cell Signaling via Stromal Reciprocation | 2016 | Publication | Phosphopeptide enrichment | Ti-IMAC, TiO2 | Mammalian cells | Automated | Chaotrope | Filter-based | Trp | Isobaric-tagging |
The co-immobilization of P450-type nitric oxide reductase and glucose dehydrogenase for the continuous reduction of nitric oxide via cofactor recycling | 2016 | Publication | Immobilization | Amine | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
The co-immobilization of P450-type nitric oxide reductase and glucose dehydrogenase for the continuous reduction of nitric oxide via cofactor recycling | 2016 | Publication | Immobilization | Carboxyl | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
An Unbiased Mass Spectrometry Approach Identifies Glypican-3 as an Interactor of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR) in Hepatocellular Carcinoma Cells | 2016 | Publication | Affinity capture | Protein G | Mammalian cells | Manual | Native | Gel-based | Trp | N/A |
Phosphoproteomic analysis of interacting tumor and endothelial cells identifies regulatory mechanisms of transendothelial migration | 2016 | Publication | Affinity capture | Protein A | Mammalian cells | Manual | Detergent | N/A | N/A | N/A |
Comprehensive protein sequence coverage using MagReSyn® Trypsin & MagReSyn® Chymotrypsin | 2016 | Application note | Digest preparation | Trypsin | Recombinant protein | Automated | Chaotrope | In-solution | Trp | Label-free |
Comprehensive protein sequence coverage using MagReSyn® Trypsin & MagReSyn® Chymotrypsin | 2016 | Application note | Digest preparation | Chymotrypsin | Recombinant protein | Automated | Chaotrope | In-solution | ChymoTrp | Label-free |
Improved stability of MagReSyn® Trypsin in the presence of denaturants: SDS and Urea | 2016 | Application note | Digest preparation | Trypsin | Recombinant protein | Manual | Chaotrope | In-solution | Trp | Label-free |
Efficient purification of polyhistidinetagged proteins under denaturing conditions using MagReSyn® NTA Inclusion bodies & hidden tags | 2015 | Application note | Protein Purification | Ni-NTA | Bacterial cells | Manual | Chaotrope | N/A | N/A | N/A |
MagReSyn NTA Increased purity of histidine-tagged proteins through enhanced binding affinity | 2015 | Application note | Protein Purification | Ni-NTA | Bacterial cells | Manual | Native | N/A | N/A | N/A |
Product Guide MagReSyn® NTA Screening Kit | 2015 | Method | Protein Purification | NTA-Kit | Bacterial cells | Manual | Native | N/A | N/A | N/A |
Product Guide MagReSyn® NTA | 2015 | Method | Protein Purification | Ni-NTA | Bacterial cells | Manual | Native | N/A | N/A | N/A |
A colorimetric-based amplification system for proteinases including MMP2 and ADAM8 | 2015 | Publication | Immobilization | Ni-NTA | Mammalian cells | Manual | Native | N/A | N/A | N/A |
Product Guide MagReSyn® SAX | 2015 | Method | Protein Purification | SAX | N/A | Manual | Native | N/A | N/A | N/A |
Immobilization of thermostable nucleoside phosphorylases on MagReSyn® epoxide microspheres and their application for the synthesis of 2,6-dihalogenated purine nucleosides | 2015 | Publication | Immobilization | Amine | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
Synthesis of 2,6-Dihalogenated Purine Nucleosides by Thermostable Nucleoside Phosphorylases | 2015 | Publication | Immobilization | Amine | Recombinant protein | Manual | N/A | N/A | N/A | N/A |
Ultrasensitive proteome analysis using paramagnetic bead technology | 2015 | Publication | Digest preparation | Carboxyl | Drosophila | Manual | Detergent | Precipitation on-bead | Trp | Label-free |
Reproducible Automated Phosphopeptide Enrichment Using Magnetic TiO2 and Ti-IMAC | 2014 | Publication | Phosphopeptide enrichment | Ti-IMAC, TiO2 | Mammalian cells | Automated | N/A | Filter-based | LysC, Trp | Metabolic labelling |
Cell-specific Labeling Enzymes for Analysis of CellCell Communication in Continuous Co-culture | 2014 | Publication | Phosphopeptide enrichment | Ti-IMAC, TiO2 | Mammalian cells | Manual | Chaotrope | Filter-based | LysC, Trp | Metabolic labelling |
Spectrophotometric activity microassay for pure and recombinant cytochrome P450-type nitric oxide reductase | 2014 | Publication | Protein Purification | Ni-NTA | Bacterial cells | Manual | Detergent | N/A | N/A | N/A |
Robotic Platform for Parallelized Cultivation and Monitoring of Microbial Growth Parameters in Microwell Plates | 2014 | Publication | Protein Purification | Ni-NTA | Bacterial cells | Automated | Native | N/A | N/A | N/A |
Effective immunotherapy of rat glioblastoma with prolonged intratumoral delivery of exogenous heat shock protein Hsp70 | 2014 | Publication | Affinity capture | Streptavidin | Mammalian tissue | Manual | Native | In-solution | N/A | N/A |
PASTA kinase-dependent control of peptidoglycan synthesis via ReoM is required for cell wall | 2021 | Publication | Phosphopeptide enrichment | TiO2, Zr-IMAC | Bacterial cells | Manual | Detergent | Precipitation in-solution | Trypsin | Label-free |
Multi-omic analysis reveals the biochemical changes underpinning the varied phenotypes of the Arabidopsis long-period mutant rve 4 6 8 | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Automated | Detergent | Precipitation on-bead | Trp | Lable-free |
A heparan sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells | 2021 | Publication | Affinity capture | Streptavidin | Mammalian cells | Manual | Detergent | Affinity capture | LysC, Trp | Label-free |
A complete and automated sample preparation strategy for high throughput and standardized proteomics | 2021 | Poster | Digest preparation | Hydroxyl | Mammalian cells | Automated | Detergent | Precipitation on-bead | LysC, Trp | Label-free |
A flexible workflow for building spectral libraries from narrow window data independent | 2021 | Publication | Digest preparation | Amine | Mammalian cells | Automated | Chaotrope | Precipitation on-bead | Trp | Label-free |
A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant Xerophyta | 2021 | Publication | Digest preparation | HILIC | Plant | Automated | Detergent | Hydrophilic affinity | Trp | Label-free |
Bromodomain factor 5 is an essential transcriptional regulator of the Leishmania genome | 2021 | Publication | BioID | Streptavidin | Mammalian cells | Manual | Detergent | Gel-based | Trp | N/A |
Early mannitol-triggered changes in the Arabidopsis leaf (phospho)proteome reveal growth | 2018 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Manual | Native | Precipitation in-solution | LysC, Trp | Label-free |
Plant Phosphoproteomics: Known Knowns, Known Unknowns, and Unknown Unknowns of an Emerging Systems Science Frontier | 2021 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | N/A | N/A | N/A | N/A | N/A |
Laboratory Protocol: Protein Aggregation Capture (PAC) | 2021 | Method | Digest preparation | Hydroxyl | N/A | Manual | Detergent | Precipitation on-bead | LysC, Trp | N/A |
Rapid factor depletion highlights intricacies of nucleoplasmic RNA degradation | 2022 | Publication | Digest preparation | Hydroxyl | Mammalian cells | Automated | Detergent | Precipitation on-bead | LysC, Trp | Isobaric-tagging |
Unraveling virus-induced cellular signaling cascades by label-free quantitative phosphoproteomics | 2022 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual, Automated | Detergent | Filter-based | Trp | Label-free |
Phosphoproteomic profiling of influenza virus entry reveals infection-triggered filopodia | 2022 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Automated | Chaotrope | Filter-based | Trp | Label-free |
Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy | 2022 | Publication | Phosphopeptide enrichment | Ti-IMAC | Mammalian cells | Manual | Subcellular fractionation | Precipitation | LysC, Trp | Isobaric-tagging |
STK25 inhibits PKA signaling by phosphorylating PRKAR1A | 2022 | Publication | Phosphopeptide enrichment | Ti-IMAC, Zr-IMAC | Mammalian cells | Manual | Chaotrope, Detergent | Precipitation | LysC, Trp | Isobaric-tagging |
Comparison of characteristics and tumor targeting properties of extracellular vesicles derived from primary NK cells or NK-cell lines stimulated with IL-15 or IL-12/15/18 | 2022 | Publication | Digest preparation | Amine | Extracellular vesicles | Manual | Detergent | Precipitation on-bead | Trp | Label-free |
Multi-omic analysis of the Arabidopsis clock activator mutant rve 4 6 8 reveals connections to carbohydrate metabolism and proteasome regulation | 2022 | Publication | Phosphopeptide enrichment | Ti-IMAC | Plant | Automated | Detergent | Precipitation on-bead | Trp | Label-free |
Systems level analysis of time and stimuli specific signaling through PKA | 2022 | Publication | Phosphopeptide enrichment | Ti-IMAC | Yeast cells | Manual | Chaotrope, Detergent | In-solution | LysC, Trp | Label-free |
Covalent coupling of Antibody to MagReSyn Carboxyl ferromagnetic microparticles using Anteobind™ | 2022 | Method | Immobilization | Carboxyl | N/A | Manual | N/A | N/A | N/A | N/A |
General Enquiries
+27 12 841 4153
+27 86 571 0854