Method Library

This resource aims to make methods more accessible to researchers, and help identify the most relevant workflows for your current application. We would appreciate your participation in keeping this database up to date, so please report any errors such as broken links, missing information, or publications that are not included to info@resynbio.com.

Resource Title	Year	Resource	Application	Product	Sample Source	Strategy	Extraction	Clean-up	Digestion	Quantification
Preparation and HILIC SPE clean-up of SDS extracted plasma proteins for MS analysis	2022	Method	Digest preparation	HILIC	Biofluids	Manual	Detergent	Hydrophilic affinity	LysC, Trp	Label-free
Preparation and HILIC SPE clean-up of urinary proteins for MS analysis	2022	Method	Digest preparation	HILIC	Biofluids	Manual	Detergent	Hydrophilic affinity	LysC, Trp	Label-free
Tissue-wide Effects Overrule Cell-intrinsic Gene Function in Cortical Projection Neuron Migration	2022	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian tissue	Manual	Kit	Kit	Trp	Isobaric-tagging
ULK4 and Fused/STK36 interact to mediate assembly of a motile flagellum	2022	Publication	BioID	Streptavidin	Mosquito cells	Manual	Detergent	Affinity capture	N/A	Label-free
Assessment of Streptavidin Bead Binding Capacity to Improve Quality of Streptavidin-based Enrichment Studies	2021	Publication	Affinity capture	Streptavidin	N/A	N/A	N/A	N/A	N/A	Label-free
Liver-fibrosis-activated transcriptional networks govern hepatocyte reprogramming and intra-hepatic communication	2021	Publication	Digest preparation	HILIC	Mammalian tissue	Manual	Chaotrope	Precipitation on-bead	LysC, Trp	Label-free
Bromodomain factor 5 is an essential transcriptional regulator of the Leishmania genome	2021	Publication	BioID	Streptavidin	Mammalian cells, Protozoa	Manual	Detergent	Affinity capture	Trp	Label-free
Dissecting the Functional Selectivity of TGF? Signaling Pathway Components Using Genome A	2021	Publication	Digest preparation, Phosphopeptide enrichment	Amine, Ti-IMAC	Mammalian tissue	Automated	Detergent	Precipitation on-bead	LysC, Trp	Isobaric-tagging
Loquacious Modulates Flaviviral RNA Replication in Mosquito Cells	2021	Publication	Affinity capture	Streptavidin	Insect cells	Manual	Detergent	Affinity capture	N/A	Label-free
Dysregulated healing responses in diabetic wounds occur in the early stages postinjury	2021	Publication	Digest preparation	HILIC	Mammalian tissue	Manual	Organic	Hydrophilic affinity	Trp	Isobaric-tagging
The cGMP-Dependent Protein Kinase 2 Contributes to Cone Photoreceptor Degeneration in the Cnga3-Deficient Mouse Model of Achromatopsia	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian tissue	Manual	N/A	Precipitation in-solution	Trp	Label-free
Taylor-made production of pyrimidine nucleoside-5′-monophosphate analogues by highly stabilized mutant uracil phosphoribosyltransferase from Toxoplasma gondii	2021	Publication	Immobilization	Amine, Ni-NTA	Enzyme	N/A	N/A	N/A	N/A	N/A
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases	2021	Publication	Immobilization	Amine	Enzyme	N/A	N/A	N/A	N/A	N/A
Spatial-proteomics reveals phospho-signaling dynamics at subcellular resolution	2021	Publication	Digest preparation, Phosphopeptide enrichment	Amine, Ti-IMAC	Mammalian tissue	Automated	Native, Detergent	Precipitation on-bead	LysC, Trp	Label-free
CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Detergent	Precipitation in-solution	Trp	Metabolic labelling
Identifying cytokine signaling signatures in primary human Th-1 cells by phospho-proteomics analysis	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Detergent	Precipitation in-solution	Trp	Metabolic labelling
Molecular roadmap of the healthy stress response in the mouse hippocampus	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian tissue	Manual	Detergent	Filter-based	Trp	Label-free
HNF4A is required to specify glucocorticoid action in the liver	2021	Publication	ChIP	Protein G	Mammalian tissue	Manual	N/A	N/A	N/A	N/A
Rapid auxin-mediated phosphorylation of Myosin regulates trafficking and polarity in Arabidopsis	2021	Publication	Phosphopeptide enrichment	TiO2, Ti-IMAC	Plant	Manual	Detergent	Hybrid	Trp	Label-free
Venom Proteomic Analysis of Two Medically Important Nigerian Viper (Echis Ocellatus and Bitis Arietans) Snake Species	2021	Publication	Digest preparation	HILIC	Snake venom	Manual	N/A	Hybrid	Trp	Label-free
Monocyte Released HERV-K dUTPase Engages TLR4 and MCAM Causing Endothelial Mesenchymal Transition	2021	Publication	Peptide clean-up	HILIC	Mammalian cells	Manual	Native	Hydrophilic affinity	LysC, Trp	Label-free
Proteomics of resistance to Notch1 inhibition in acute lymphoblastic leukemia reveals targetable kinase signatures	2021	Publication	Digest preparation, Phosphopeptide enrichment	Amine, Ti-IMAC	Mammalian cells	Automated	Detergent	Precipitation on-bead	LysC, Trp	Isobaric-tagging, Label-free
KF Method: Automated Phosphopeptide Enrichment using MagReSyn® IMAC	2021	Method	Phosphopeptide enrichment	Ti-IMAC, Zr-IMAC	N/A	Automated	N/A	N/A	N/A	Label-free
High-throughput phosphoproteomics profiling of non-small cell lung cancer tissues	2021	Poster	Phosphopeptide enrichment	Ti-IMAC	Mammalian tissue	Automated	N/A	In-solution	Trp	Label-free
The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted antiviral signaling and host translation	2021	Publication	BioID	Streptavidin	Mammalian cells	Automated	Detergent	Affinity capture	LysC, Trp	Label-free
Product Guide MagReSyn® Trypsin	2021	Method	Digest preparation	Trypsin	N/A	Manual	N/A	In-solution	Trp	N/A
Product Guide MagReSyn® Chymotrypsin	2021	Method	Digest preparation	Chymotrypsin	Recombinant protein	Manual	N/A	In-solution	ChymoTrp	N/A
Competitive binding of STATs to receptor phospho-Tyr motifs accounts for altered cytokine responses	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Detergent	Precipitation in-solution	Trp	Metabolic labelling
Drug-bound and -free outward-facing structures of a multidrug ABC exporter point to a swing mechanism	2021	Publication	HDX	Zr-IMAC	Recombinant protein	N/A	N/A	N/A	Pepsin	HDX
Antagonistic cell surface and intracellular auxin signalling regulate plasma membrane H+ fluxes for root growth	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Manual	Detergent	Filter-based	Trp	Label-free
The membrane-localized protein kinase MAP4K4/TOT3 regulates thermomorphogenesis	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Manual	Native	Precipitation in-solution	LysC, Trp	Label-free
The cGMP-Dependent Protein Kinase 2 Contributes to Cone Photoreceptor Degeneration in the Cnga3-Deficient Mouse Model of Achromatopsia	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian tissue	Manual	N/A	Precipitation in-solution	Trp	Label-free
Unraveling the MAX2 Protein Network in Arabidopsis thaliana: Identification of the Protein Phosphatase PAPP5 as a Novel MAX2 Interactor	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Manual	Native	Affinity capture	LysC, Trp	Label-free
An AKT2-specific nanobody that targets the hydrophobic motif induces cell cycle arrest, autophagy and loss of focal adhesions in MDA-MB-231 cells	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Chaotrope	In-solution	LysC, Trp	Label-free
Evaluation of Protein Purification Techniques and Effects of Storage Duration on LC-MS/MS Analysis of Archived FFPE Human CRC Tissues	2021	Publication	Digest preparation	HILIC	FFPE	Automated	Detergent	Hydrophilic affinity	Trp	Label-free
Functional studies of CpSRP54 in diatoms show that themechanism of thylakoid protein insertion differs from that inplants and green algae	2021	Publication	Digest preparation	HILIC	Mammalian cells	Automated	Detergent	Hydrophilic affinity	Trp	Label-free
Proteomic analysis of three medically important Nigerian Naja (Naja haje, Naja katiensis and Naja nigricollis) snake venoms	2021	Publication	Digest preparation	HILIC	Snake venom	Manual	N/A	Hydrophilic affinity	Trp	Label-free
Plasmodium falciparum Calcium Dependent Protein Kinase 4 is critical for male gametogenesis and transmission to the mosquito vector	2021	Publication	Phosphopeptide enrichment	Ti-IMAC, Zr-IMAC	Plasmodium	Automated	Detergent	Precipitation in-solution	Trp	Label-free
Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion	2021	Publication	Digest preparation, Phosphopeptide enrichment	HILIC, Ti-IMAC	Mammalian cells, Other	Manual	Detergent	Precipitation on-filter, Affinity capture	Trp	Isobaric-tagging, Label-free
DYRK3-Controlled Phase Separation Organizes the Early Secretory Pathway	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Automated	N/A	Other	Trp	Label-free
Phosphorylation controls RNA binding and transcription by the influenza virus polymerase	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Chaotrope	Precipitation in-solution	Trp	Label-free
Modulation of immune cell reactivity with cis-binding Siglec agonists	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Detergent	Precipitation on-filter	Trp	Isobaric-tagging
A Compact Quadrupole-Orbitrap Mass Spectrometer with FAIMS Interface Improves Proteome Coverage in Short LC Gradients	2020	Publication	Digest preparation, Phosphopeptide enrichment	Amine, Ti-IMAC	Mammalian cells, Mammalian tissue	Automated	Detergent	Precipitation on-bead	LysC, Trp	Label-free
Chemical Genetics of AGC-kinases Reveals Shared Targets of Ypk1, Protein Kinase A and Sch9	2020	Publication	Phosphopeptide enrichment	Ti-IMAC, TiO2, ZrO2	Yeast cells	Manual	N/A	Precipitation in-solution	Trp	Label-free
Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	Yeast cells	Manual	Detergent	Precipitation in-solution	LysC, Trp	Label-free
Functional studies of CpSRP54 in diatoms show that the mechanism of thylakoid protein insertion differs from that in plants and green algae	2020	Publication	Digest preparation	HILIC	Algae	Automated	Detergent	Hydrophilic affinity	Trp	Label-free
SWATH-MS based proteomic profiling of pancreatic ductal adenocarcinoma tumours reveals the interplay between the extracellular matrix and related intracellular pathways	2020	Publication	Digest preparation	HILIC	Mammalian tissue	Automated	Detergent	Hydrophilic affinity	Trp	Label-free
CDK8 fine-tunes IL-6 transcriptional activities by limiting STAT3 resident time at the gene loci	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Manual	Native	Precipitation in-solution	LysC, Trp	Label-free
The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Manual	Native	Precipitation in-solution	LysC, Trp	Label-free
Alterations in the CO2 availability induce alterations in the phosphoproteome of the cyanobacterium Synechocystis sp. PCC 6803	2020	Publication	Phosphopeptide enrichment	Ti-IMAC, TiO2	Bacterial cells	Manual	Detergent	Precipitation in-solution	LysC, Trp	Label-free
CYCA3;4 Is a Post-Prophase Target of the APC/CCCS52A2 E3-Ligase Controlling Formative Cell Divisions in Arabidopsis	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Detergent	N/A	Trp	Metabolic labelling
HIPK4 is essential for murine spermiogenesis	2020	Publication	Phosphopeptide enrichment	Ti-IMAC, TiO2	Mammalian cells	Manual	Detergent	Precipitation in-solution	LysC, Trp	Isobaric-tagging
Fluorescence fluctuation analysis reveals PpV dependent Cdc25 protein dynamics in living embryos	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	Drosophila	Manual	Native	Gel-based	Trp (in-gel)	Label-free
Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Automated	Detergent	Precipitation in-solution	LysC, Trp	Isobaric-tagging
Cannabinoid exposure in rat adolescence reprograms the initial behavioral, molecular, and epigenetic response to cocaine	2020	Publication	Phosphopeptide enrichment	Ti-IMAC, Zr-IMAC	Mammalian tissue	Manual	Chaotrope	Precipitation in-solution	LysC, Trp	Isobaric-tagging
The Diabetes Gene JAZF1 Is Essential for the Homeostatic Control of Ribosome Biogenesis and Function in Metabolic Stress	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Detergent	Filter-based	N/A	Label-free
Phosphoproteomic Effects of Acute Depletion of PP2A Regulatory Subunit Cdc	2020	Publication	Phosphopeptide enrichment	Ti-IMAC, TiO2, ZrO2	Yeast cells	Manual	Chaotrope	In-solution	LysC, Trp	Metabolic labelling
An Unbiased Approach to Mapping the Signaling Network of the Pseudorabies Virus US3 Protein	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Chaotrope	In-solution	LysC, Trp	Label-free
Zirconium(IV)-IMAC Revisited: Improved Performance and Phosphoproteome Coverage by Magnetic Microparticles for Phosphopeptide Affinity Enrichment	2020	Publication	Phosphopeptide enrichment	Ti-IMAC, Zr-IMAC, TiO2	Mammalian cells	Both	Detergent	Precipitation in-solution	Trp	Label-free
Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in translation	2020	Publication	Phosphopeptide enrichment	TiO2	Plant	Manual	Native	In-solution	Trp	Isobaric-tagging
Magnetic micro-macro biocatalysts applied to industrial bioprocesses	2020	Publication	Immobilization	Ni-NTA	Recombinant protein	Manual	N/A	N/A	N/A	N/A
Magnetic micro-macro biocatalysts applied to industrial bioprocesses	2020	Publication	Immobilization	Amine	Recombinant protein	Manual	N/A	N/A	N/A	N/A
Novel kinase platform for the validation of the anti-tubercular activities of Pelargonium sidoides (Geraniaceae)	2020	Publication	Protein Purification	Ni-NTA	Bacterial cells	Manual	Native	N/A	N/A	N/A
Product Guide MagReSyn® TiO2	2020	Method	Phosphopeptide enrichment	TiO2	N/A	Manual	N/A	N/A	N/A	Label-free
Evaluation of Novel Supports for Selective and Efficient Enrichment of Phosphorylated Peptides	2020	Method	Phosphopeptide enrichment	TiO2, ZrO2	N/A	Automated	N/A	N/A	N/A	Label-free
Product Guide MagReSyn® ZrO2	2020	Method	Phosphopeptide enrichment	ZrO2	N/A	Manual	N/A	N/A	N/A	Label-free
Product Guide MagReSyn® Ti-IMAC HP	2020	Method	Phosphopeptide enrichment	Ti-IMAC	N/A	Manual	N/A	N/A	N/A	Label-free
Product Guide MagReSyn® Ti-IMAC	2020	Method	Phosphopeptide enrichment	Ti-IMAC	N/A	Manual	N/A	N/A	N/A	Label-free
A Data Independent Acquisition Workflow Enables the Profiling of Thousands of Human Cancer Tissues for Precision Oncology	2020	Poster	Phosphopeptide enrichment	Ti-IMAC	Mammalian tissue	Automated	Unknown	In-solution	Trp	Label-free
Fast and reproducible phosphoproteomics using MagReSyn® Amine and Ti-IMAC HP magnetic beads and the Evosep One	2020	Application note	Digest preparation, Phosphopeptide enrichment	Amine, Ti-IMAC	Mammalian cells	Automated	Detergent	Precipitation on-bead	LysC, Trp	Label-free
High Throughput Proteome and Phosphoproteome Sample Processing Couple to Fast Gradient DIA	2020	Poster	Phosphopeptide enrichment	Ti-IMAC	Mammalian tissue	Automated	Detergent	Precipitation on-bead	LysC, Trp	Label-free
High Throughput Proteome and Phosphoproteome Sample Processing Couple to Fast Gradient DIA	2020	Poster	Digest preparation	Amine	Mammalian tissue	Automated	Detergent	Precipitation on-bead	LysC, Trp	Label-free
Product Guide MagReSyn® Zr-IMAC HP	2020	Method	Phosphopeptide enrichment	Zr-IMAC	N/A	Manual	N/A	N/A	N/A	Label-free
Product Guide MagReSyn® Zr-IMAC	2020	Method	Phosphopeptide enrichment	Zr-IMAC	N/A	Manual	N/A	N/A	N/A	Label-free
Zirconium(IV) IMAC-based enrichment for mass spectrometry driven phosphoproteomics	2020	Poster	Phosphopeptide enrichment	Ti-IMAC, Zr-IMAC	Mammalian cells	Automated	N/A	N/A	N/A	Label-free
KF Method: Automated clean-up and on-bead digestion with MagReSyn® HILIC	2020	Method	Digest preparation	HILIC	N/A	Automated	Detergent	Hydrophilic affinity	Trp	Label-free
Sustainable synthesis of uridine-5?-monophosphate analogues by immobilized uracil phosphoribosyltransferase from Thermus thermophilus	2020	Publication	Immobilization	Amine	Recombinant protein	Manual	N/A	N/A	N/A	N/A
Identification of MAMP-Responsive Plasma Membrane-Associated Proteins in Arabidopsis thaliana Following Challenge with Different LPS Chemotypes from Xanthomonas campestris	2020	Publication	Affinity capture	Streptavidin	Bacterial cells	Manual	N/A	N/A	N/A	N/A
Biochemical Pulldown of mRNAs and Long Noncoding RNAs from Cellular Lysates Coupled with Mass Spectrometry to Identify Protein Binding Partners	2020	Publication	Affinity capture	Streptavidin	Mammalian cells	Manual	Detergent	Gel-based	Trp	Label-free
Multiplexed Proximity Biotinylation Coupled to Mass Spectrometry for Defining Integrin Adhesion Complexes	2020	Publication	BioID	Streptavidin	Mammalian cells	Manual	Detergent	Gel-based	Trp	Label-free
Topological features of integrin adhesion complexes revealed by multiplexed proximity biotinylation	2020	Publication	BioID	Streptavidin	Mammalian cells	Manual	Detergent	Gel-based	Trp	Label-free
BioID based proteomic analysis of the Bid interactome identifies novel proteins involved in cell cycle dependent apoptotic priming	2020	Publication	BioID	Streptavidin	Mammalian cells	Manual	Detergent	Gel-based	Trp	Label-free
Plant-based production of highly potent anti-HIV antibodies with engineered posttranslational modifications	2020	Publication	Affinity capture	Protein A	Plant	Manual	Native	N/A	N/A	N/A
Hypoxia regulates GR function through multiple mechanisms involving microRNAs 103 and 107	2020	Publication	ChIP	Protein G	Mammalian cells	Manual	N/A	N/A	N/A	qPCR
Insights from the First Phosphopeptide Challenge of the MS Resource Pillar of the HUPO Human Proteome Project	2020	Publication	Phosphopeptide enrichment	Ti-IMAC	HUPO Synthetic	Both	N/A	N/A	N/A	N/A
Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis	2019	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian tissue	Manual	Chaotrope	Precipitation in-solution	Trp double digest	Isobaric-tagging
Proteasome-mediated remodeling of the proteome and phosphoproteome during kiwifruit pollen germination	2019	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Manual	N/A	Hybrid	N/A	Label-free
Conduit integrity is compromised during acute lymph node expansion	2019	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Automated	Chaotrope	Filter-based	N/A	Isobaric-tagging
Probability-based detection of phosphoproteomic uncertainty reveals rare signaling events driven by oncogenic kinase gene fusion	2019	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Detergent	Precipitation in-solution	Trp	Label-free
CDK8 Regulates Insulin Secretion and Mediates Postnatal and Stress-Induced Expression of Neuropeptides in Pancreatic ? Cells	2019	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Detergent	Filter-based	N/A	Label-free
Protein Aggregation Capture on Microparticles Enables Multipurpose Proteomics Sample Preparation	2019	Publication	Digest preparation, Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Detergent	Precipitation on-bead	LysC, Trp	Label-free
R2-P2 rapid-robotic phosphoproteomics enables multidimensional cell signaling studies	2019	Publication	Phosphopeptide enrichment	Ti-IMAC, Zr-IMAC, TiO2	Yeast cells	Automated	Chaotrope	Precipitation on-bead	LysC, Trp	Label-free
Development of fully automated pipeline for phosphoproteome profiling optimisation of phosphopeptide enrichment	2019	Poster	Phosphopeptide enrichment	Ti-IMAC, Zr-IMAC, TiO2, ZrO2, Amine, HILIC, SAX	Recombinant protein, Mammalian cells	Automated	Detergent, Chaotrope	In-solution, Precipitation on-bead, Hydrophilic affinity, Ion exchange	Trp	Isotope-labelled peptides, Label-free
Development of a fully automated high throughput magnetic workflow for phosphoproteome profiling	2019	Poster	Phosphopeptide enrichment	Ti-IMAC, Zr-IMAC, TiO2	Mammalian cells	Automated	Detergent	Precipitation on-bead	Trp	Label-free
Development of fully automated pipeline for phosphoproteome profiling	2019	Poster	Digest preparation, Phosphopeptide enrichment	Ti-IMAC, SAX, HILIC	Mammalian cells	Automated	Detergent, Chaotrope	Precipitation on-bead, Hydrophilic affinity, Ion exchange	Trp	Label-free
PEPCLU MagReSyn HILIC Clean-Up of Peptides Post Protein Digestion	2019	Method	Peptide clean-up	HILIC	N/A	Manual	Detergent	Hydrophilic affinity	N/A	Label-free
RAPOBD HILIC Clean-Up of Reduced & Alkylated Proteins with on Bead Digestion	2019	Method	Digest preparation	HILIC	Mammalian cells	Manual	Detergent	Hydrophilic affinity	Trp	Label-free
POBRAD HILIC Clean-Up of proteins with on-bead reduction, alkylation and digestion	2019	Method	Digest preparation	HILIC	Mammalian cells	Manual	Detergent	Hydrophilic affinity	Trp	Label-free
Improved reproducibility of automated sample preparation using HILIC magnetic beads automated by Hamilton Robotics	2019	Application note	Digest preparation	HILIC	N/A	Automated	Detergent	Hydrophilic affinity	Trp	Label-free
Development of a fully automated magnetic workflow for phosphoproteome profiling	2019	Poster	Digest preparation, Phosphopeptide enrichment	Ti-IMAC, Zr-IMAC, TiO2, ZrO2, SAX, HILIC	Mammalian cells	Automated	Detergent, Chaotrope	Hydrophilic affinity, Ion exchange	Trp	Label-free
Product Guide MagResyn® Amine - activation with epoxide for protein immobilization	2019	Method	Immobilization	Amine	N/A	Manual	N/A	N/A	N/A	N/A
Product Guide MagReSyn® Streptavidin	2019	Method	Affinity capture	Streptavidin	N/A	Manual	N/A	N/A	N/A	N/A
Product Guide MagReSyn® Streptavidin MAX	2019	Method	Affinity capture	Streptavidin	N/A	Manual	N/A	N/A	N/A	N/A
Product Guide MagReSyn® Protein A	2019	Method	Affinity capture	Protein A	N/A	Manual	N/A	N/A	N/A	N/A
Product Guide MagReSyn® Protein A MAX	2019	Method	Affinity capture	Protein A	N/A	Manual	N/A	N/A	N/A	N/A
Product Guide MagReSyn® Protein G	2019	Method	Affinity capture	Protein G	N/A	Manual	N/A	N/A	N/A	N/A
p38α MAPK proximity assay reveals a regulatory mechanism of alternative splicing in cardiomyocytes	2019	Publication	Affinity capture	Protein G	Mammalian tissue	Manual	Detergent	Gel-based	N/A	N/A
An improved method for quantitative ChIP studies of nuclear receptor function	2019	Publication	ChIP	Protein G	Mammalian tissue	Manual	N/A	In-solution	N/A	qPCR
The Auxin-Regulated CrRLK1L Kinase ERULUS Controls Cell Wall Composition during Root Hair Tip Growth	2018	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Manual	Native	Precipitation in-solution	LysC, Trp	Label-free
Ultra-High Pressure (>30,000 psi) Packing of Capillary Columns Enhancing Depth of Shotgun Proteomic Analyses	2018	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells, Mammalian tissue	Manual	Chaotrope	Precipitation in-solution	LysC, Trp	Isobaric-tagging
Magnetic HILIC: An enabling & versatile tool for robust automated MS sample preparation workflows	2018	Poster	Digest preparation	HILIC	Mammalian cells	Automated	Detergent	Hydrophilic affinity	Trp	Label-free
Magnetic HILIC: An enabling & versatile tool for robust automated MS sample preparation workflows	2018	Poster	Peptide clean-up	HILIC	Mammalian cells	Automated	Detergent	Hydrophilic affinity	Trp	Label-free
Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives	2018	Publication	Immobilization	Amine	Recombinant protein	Manual	N/A	N/A	N/A	N/A
Enzymatic Synthesis of Therapeutic Nucleosides using a Highly Versatile Purine Nucleoside 2-DeoxyribosylTransferase from Trypanosoma brucei	2018	Publication	Immobilization	Amine	Recombinant protein	Manual	N/A	N/A	N/A	N/A
One-Pot, One-Step Production of Dietary Nucleotides by Magnetic Biocatalysts	2018	Publication	Immobilization	Amine	Recombinant protein	Manual	N/A	N/A	N/A	N/A
Immune genes are primed for robust transcription by proximal long noncoding RNAs located in nuclear compartments	2018	Publication	Affinity capture	Streptavidin, Protein A, Protein G	Mammalian cells	Manual	Detergent	Gel-based	N/A	qPCR
REVERBa couples the circadian clock to hepatic glucocorticoid action	2018	Publication	ChIP, Affinity capture	Protein A, Protein G	Mammalian cells	Manual	N/A	N/A	N/A	qPCR
LRP1 is required for novobiocin-mediated fibronectin turnover	2018	Publication	Affinity capture	Protein A	Mammalian cells	Manual	Detergent	Gel-based	Trp	N/A
Circadian clock component REV-ERB? controls homeostatic regulation of pulmonary inflammation	2018	Publication	Affinity capture	Protein A	Mammalian cells	Manual	Detergent	Gel-based	Trp	N/A
Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation	2017	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Automated	Detergent	Precipitation in-solution	Trp, AspN	Isobaric-tagging
Phosphoproteomics with Activated Ion Electron Transfer Dissociation	2017	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian tissue	Manual	Chaotrope	Precipitation in-solution	Trp	Label-free
Proteome Profiling of Wheat Shoots from Different Cultivars	2017	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Manual	Native	Precipitation in-solution	LysC, Trp	Label-free
GSK3α and GSK3β Phosphorylate Arc and Regulate its Degradation	2017	Publication	Phosphopeptide enrichment	Ti-IMAC	Recombinant protein	Manual	Detergent	Other	Trp, Pepsin	Label-free
Identification of ADP-Ribose Acceptor Sites on In Vitro Modified Proteins by Liquid ChromatographyTandem Mass Spectrometry	2017	Publication	ADP-ribosylation enrichment	Ti-IMAC	Recombinant protein	Manual	Chaotrope	Filter-based	Trp	Label-free
Cell-Specific Labeling for Analyzing Bidirectional Signaling by Mass Spectrometry	2017	Publication	Phosphopeptide enrichment	Ti-IMAC, TiO2	Mammalian cells	Manual	Chaotrope	Filter-based	Trp	Metabolic labelling
Comparative genetic, proteomic and phosphoproteomic analysis of C. elegans embryos with a focus on ham-1/STOX and pig-1/MELK in dopaminergic neuron development	2017	Publication	Phosphopeptide enrichment	Ti-IMAC	C. elegans	Automated	Chaotrope	In-solution	LysC, Trp	Label-free
HIV-1 Activates T Cell Signaling Independently of Antigen to Drive Viral Spread	2017	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Detergent	Filter-based	Trp double digest	Metabolic labelling
Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS	2017	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Detergent	Precipitation on-bead	LysC, Trp	Isobaric-tagging
Reactivation of MagReSyn® Ti-IMAC	2017	Method	Phosphopeptide enrichment	Ti-IMAC	N/A	N/A	N/A	N/A	N/A	N/A
Product Guide MagReSyn® HILIC	2017	Method	Digest preparation	HILIC	N/A	Manual	Detergent	Hydrophilic affinity	Trp	Label-free
Universal Unbiased pre-MS Clean-Up Using Magnetic HILIC Microparticles for SPE	2017	Poster	Digest preparation	HILIC	Mammalian cells	Automated	Detergent	Hydrophilic affinity	Trp	Label-free
Dealing with the Dirty Dozen: Universal Unbiased preMS Clean-Up Using Magnetic HILIC SPE	2017	Poster	Digest preparation	HILIC	Mammalian cells	Automated	Detergent	Hydrophilic affinity	Trp	Label-free
Product Guide MagReSyn® Amine	2017	Method	Immobilization	Amine	N/A	Manual	N/A	N/A	N/A	N/A
Structural and functional characterization of thermostable biocatalysts for the synthesis of 6-aminopurine nucleoside-5?-monophospate analogues	2017	Publication	Immobilization	Amine	Recombinant protein	Manual	N/A	N/A	N/A	N/A
Product Guide MagReSyn® Carboxyl	2017	Method	Immobilization	Carboxyl	Recombinant protein	Manual	N/A	N/A	N/A	N/A
Identification of lipopolysaccharide-interacting plasma membrane-type proteins in Arabidopsis thaliana	2017	Publication	Affinity capture	Streptavidin	Plant	Manual	Native	Gel-based	Trp	N/A
Up-to-Date Workflow for Plant (Phospho)proteomics Identifies Differential Drought-Responsive Phosphorylation Events in Maize Leaves	2016	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Manual	Native	Precipitation in-solution	LysC, Trp	Label-free
Oncogenic KRAS Regulates Tumor Cell Signaling via Stromal Reciprocation	2016	Publication	Phosphopeptide enrichment	Ti-IMAC, TiO2	Mammalian cells	Automated	Chaotrope	Filter-based	Trp	Isobaric-tagging
The co-immobilization of P450-type nitric oxide reductase and glucose dehydrogenase for the continuous reduction of nitric oxide via cofactor recycling	2016	Publication	Immobilization	Amine	Recombinant protein	Manual	N/A	N/A	N/A	N/A
The co-immobilization of P450-type nitric oxide reductase and glucose dehydrogenase for the continuous reduction of nitric oxide via cofactor recycling	2016	Publication	Immobilization	Carboxyl	Recombinant protein	Manual	N/A	N/A	N/A	N/A
An Unbiased Mass Spectrometry Approach Identifies Glypican-3 as an Interactor of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR) in Hepatocellular Carcinoma Cells	2016	Publication	Affinity capture	Protein G	Mammalian cells	Manual	Native	Gel-based	Trp	N/A
Phosphoproteomic analysis of interacting tumor and endothelial cells identifies regulatory mechanisms of transendothelial migration	2016	Publication	Affinity capture	Protein A	Mammalian cells	Manual	Detergent	N/A	N/A	N/A
Comprehensive protein sequence coverage using MagReSyn® Trypsin & MagReSyn® Chymotrypsin	2016	Application note	Digest preparation	Trypsin	Recombinant protein	Automated	Chaotrope	In-solution	Trp	Label-free
Comprehensive protein sequence coverage using MagReSyn® Trypsin & MagReSyn® Chymotrypsin	2016	Application note	Digest preparation	Chymotrypsin	Recombinant protein	Automated	Chaotrope	In-solution	ChymoTrp	Label-free
Improved stability of MagReSyn® Trypsin in the presence of denaturants: SDS and Urea	2016	Application note	Digest preparation	Trypsin	Recombinant protein	Manual	Chaotrope	In-solution	Trp	Label-free
Efficient purification of polyhistidinetagged proteins under denaturing conditions using MagReSyn® NTA Inclusion bodies & hidden tags	2015	Application note	Protein Purification	Ni-NTA	Bacterial cells	Manual	Chaotrope	N/A	N/A	N/A
MagReSyn NTA Increased purity of histidine-tagged proteins through enhanced binding affinity	2015	Application note	Protein Purification	Ni-NTA	Bacterial cells	Manual	Native	N/A	N/A	N/A
Product Guide MagReSyn® NTA Screening Kit	2015	Method	Protein Purification	NTA-Kit	Bacterial cells	Manual	Native	N/A	N/A	N/A
Product Guide MagReSyn® NTA	2015	Method	Protein Purification	Ni-NTA	Bacterial cells	Manual	Native	N/A	N/A	N/A
A colorimetric-based amplification system for proteinases including MMP2 and ADAM8	2015	Publication	Immobilization	Ni-NTA	Mammalian cells	Manual	Native	N/A	N/A	N/A
Product Guide MagReSyn® SAX	2015	Method	Protein Purification	SAX	N/A	Manual	Native	N/A	N/A	N/A
Immobilization of thermostable nucleoside phosphorylases on MagReSyn® epoxide microspheres and their application for the synthesis of 2,6-dihalogenated purine nucleosides	2015	Publication	Immobilization	Amine	Recombinant protein	Manual	N/A	N/A	N/A	N/A
Synthesis of 2,6-Dihalogenated Purine Nucleosides by Thermostable Nucleoside Phosphorylases	2015	Publication	Immobilization	Amine	Recombinant protein	Manual	N/A	N/A	N/A	N/A
Ultrasensitive proteome analysis using paramagnetic bead technology	2015	Publication	Digest preparation	Carboxyl	Drosophila	Manual	Detergent	Precipitation on-bead	Trp	Label-free
Reproducible Automated Phosphopeptide Enrichment Using Magnetic TiO2 and Ti-IMAC	2014	Publication	Phosphopeptide enrichment	Ti-IMAC, TiO2	Mammalian cells	Automated	N/A	Filter-based	LysC, Trp	Metabolic labelling
Cell-specific Labeling Enzymes for Analysis of CellCell Communication in Continuous Co-culture	2014	Publication	Phosphopeptide enrichment	Ti-IMAC, TiO2	Mammalian cells	Manual	Chaotrope	Filter-based	LysC, Trp	Metabolic labelling
Spectrophotometric activity microassay for pure and recombinant cytochrome P450-type nitric oxide reductase	2014	Publication	Protein Purification	Ni-NTA	Bacterial cells	Manual	Detergent	N/A	N/A	N/A
Robotic Platform for Parallelized Cultivation and Monitoring of Microbial Growth Parameters in Microwell Plates	2014	Publication	Protein Purification	Ni-NTA	Bacterial cells	Automated	Native	N/A	N/A	N/A
Effective immunotherapy of rat glioblastoma with prolonged intratumoral delivery of exogenous heat shock protein Hsp70	2014	Publication	Affinity capture	Streptavidin	Mammalian tissue	Manual	Native	In-solution	N/A	N/A
PASTA kinase-dependent control of peptidoglycan synthesis via ReoM is required for cell wall	2021	Publication	Phosphopeptide enrichment	TiO2, Zr-IMAC	Bacterial cells	Manual	Detergent	Precipitation in-solution	Trypsin	Label-free
Multi-omic analysis reveals the biochemical changes underpinning the varied phenotypes of the Arabidopsis long-period mutant rve 4 6 8	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Automated	Detergent	Precipitation on-bead	Trp	Lable-free
A heparan sulfate-bearing syndecan-1 glycoform is a distinct surface marker for intra-tumoral myeloid-derived suppressor cells	2021	Publication	Affinity capture	Streptavidin	Mammalian cells	Manual	Detergent	Affinity capture	LysC, Trp	Label-free
A complete and automated sample preparation strategy for high throughput and standardized proteomics	2021	Poster	Digest preparation	Hydroxyl	Mammalian cells	Automated	Detergent	Precipitation on-bead	LysC, Trp	Label-free
A flexible workflow for building spectral libraries from narrow window data independent	2021	Publication	Digest preparation	Amine	Mammalian cells	Automated	Chaotrope	Precipitation on-bead	Trp	Label-free
A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant Xerophyta	2021	Publication	Digest preparation	HILIC	Plant	Automated	Detergent	Hydrophilic affinity	Trp	Label-free
Bromodomain factor 5 is an essential transcriptional regulator of the Leishmania genome	2021	Publication	BioID	Streptavidin	Mammalian cells	Manual	Detergent	Gel-based	Trp	N/A
Early mannitol-triggered changes in the Arabidopsis leaf (phospho)proteome reveal growth	2018	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Manual	Native	Precipitation in-solution	LysC, Trp	Label-free
Plant Phosphoproteomics: Known Knowns, Known Unknowns, and Unknown Unknowns of an Emerging Systems Science Frontier	2021	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	N/A	N/A	N/A	N/A	N/A
Laboratory Protocol: Protein Aggregation Capture (PAC)	2021	Method	Digest preparation	Hydroxyl	N/A	Manual	Detergent	Precipitation on-bead	LysC, Trp	N/A
Rapid factor depletion highlights intricacies of nucleoplasmic RNA degradation	2022	Publication	Digest preparation	Hydroxyl	Mammalian cells	Automated	Detergent	Precipitation on-bead	LysC, Trp	Isobaric-tagging
Unraveling virus-induced cellular signaling cascades by label-free quantitative phosphoproteomics	2022	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual, Automated	Detergent	Filter-based	Trp	Label-free
Phosphoproteomic profiling of influenza virus entry reveals infection-triggered filopodia	2022	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Automated	Chaotrope	Filter-based	Trp	Label-free
Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy	2022	Publication	Phosphopeptide enrichment	Ti-IMAC	Mammalian cells	Manual	Subcellular fractionation	Precipitation	LysC, Trp	Isobaric-tagging
STK25 inhibits PKA signaling by phosphorylating PRKAR1A	2022	Publication	Phosphopeptide enrichment	Ti-IMAC, Zr-IMAC	Mammalian cells	Manual	Chaotrope, Detergent	Precipitation	LysC, Trp	Isobaric-tagging
Comparison of characteristics and tumor targeting properties of extracellular vesicles derived from primary NK cells or NK-cell lines stimulated with IL-15 or IL-12/15/18	2022	Publication	Digest preparation	Amine	Extracellular vesicles	Manual	Detergent	Precipitation on-bead	Trp	Label-free
Multi-omic analysis of the Arabidopsis clock activator mutant rve 4 6 8 reveals connections to carbohydrate metabolism and proteasome regulation	2022	Publication	Phosphopeptide enrichment	Ti-IMAC	Plant	Automated	Detergent	Precipitation on-bead	Trp	Label-free
Systems level analysis of time and stimuli specific signaling through PKA	2022	Publication	Phosphopeptide enrichment	Ti-IMAC	Yeast cells	Manual	Chaotrope, Detergent	In-solution	LysC, Trp	Label-free
Covalent coupling of Antibody to MagReSyn Carboxyl ferromagnetic microparticles using Anteobind™	2022	Method	Immobilization	Carboxyl	N/A	Manual	N/A	N/A	N/A	N/A

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