Confocal microscope images of microsphere cross-sections showing the degree of penetration of fluorescently labelled BSA onto solid, cracked and ReSyn microparticles (left to right). ReSyn microparticles allow penetration of biomolecules throughout the volume of the microparticle as opposed to surface-based attachment.

Technology Description

New miniaturized, high throughput technologies for bioseparation, diagnostics, DNA sequencing, flow cytometry, drug discovery, proteomics and genomics are in many instances reliant on attachment of functional biological molecules to a microsphere support. The vast array of life sciences applications include: capture reagents for immunoassay (fluorescence, enzyme linked etc); surfaces for immunoprecipitation; diagnostic assays; fluorescence microscopy; flow/imaging cytometry; magnetic cell separation; molecular diagnostics; agglutination tests; nucleic acid separation and protein separation among others.

Conventional microparticle technologies use solid or porous/cracked microparticles with binding of biological molecules limited to the surface, a key factor constraining performance and the development of new applications for microparticle technologies and products. We have developed a novel (patented) microparticle technology platform, comprising a hyper-porous polymer matrix that allows penetration of biological and synthetic molecules throughout the volume of the microparticles.This offers exceptionally high surface area for binding of molecules and allows performance that is orders of magnitude greater than alternate technologies. The binding capacity serves as a major performance contributor to the number of applications and versatility for end-user applications by and further enables miniaturization, increased sensitivity and reducing the cost of R&D.

ReSyn Biosciences has an exclusive license to the proprietary polymer microparticle technology developed and patented at the CSIR (WO2009057049; IN267234; US9574054; ES2595104; EP2205662; DK2205662; CN101874061; CA2703478; JP五四四二六二四). ReSyn Biosciences (Pty) Ltd is a biotechnology company founded by the technology inventors.

ReSyn Technology Performance Superiority

Why choose ReSyn?

  Demonstrated performance superiority

 Technology flexibility allows unique, customizable solutions

  Readily integrated into existing workflows

  Validated automated protocols for a range of robotic platforms

Technical advantages of ReSyn for bioseparation:

  • High biomolecule binding capacity (enables miniaturization and high-throughput processes)
  • High functional group density of 3500 µmoles.g-1 vs 300-600 µmoles.g-1 for competitor technologies, allows for multi-point covalent attachment of bioseparation ligand reducing leaching and enhancing stability.
  • Improved biomolecule purity and recovery

Technical advantages of ReSyn for biomolecule immobilization:

  • High protein binding capacity (>100% protein loading)
  • Enhanced protein stability through multipoint attachment
  • Demonstrated enzyme activity retention
  • Co-immobilization of multiple enzymes
  • Immobilization of multimeric enzymes

Unique features of MagReSyn® microparticles

Resistant to oxidation

  • Increased stability and shelf-life
  • Reduced sample contamination
  • Improved reproducibility

Rapid recovery by magnetization (<10 sec)

  • Improved research efficiency
  • Reduced experimental time
  • High throughput automation compatible
  • Reduced protocol times

Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.