For rapid, automated tryptic digestion of proteins for mass spectrometry sample preparation
MagReSyn® Trypsin microparticles consist of covalently immobilized sequencing grade trypsin (TPCK treated to prevent chymotryptic activity) for protein digestion prior to mass spectrometry analysis. The porous high capacity MagReSyn® microparticle technology allows for high volumetric enzyme activity allowing for rapid digestion. The magnetically immobilized trypsin is stabilized against denaturation and retains functionality under denaturing conditions (including the presence of urea and SDS) with limited trypsin autolysis, allowing for new automated and miniaturized digestion workflows, improving digestion reproducibility.
Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Digestion ratio: 20 µl (300 µg) microspheres per 50 µg protein sample
Bead size: ~5-10 µm
Formulation: 15 mg.ml-1 suspension in 50 mM acetic acid
MagReSyn® Trypsin Performance Superiority
- High Trypsin content ensures rapid digestion
Improved stability and compatibility
- Multipoint covalent attachment of trypsin
- Low trypsin leaching reduces sample contamination
- Stability under denaturing conditions e.g. Urea and SDS allows simplification of sample preparation workflows
- Suitable for automated digestion on magnetic handling stations
- Reduce sample preparation variability and improve reproducibility
Digestion efficiency of samples containing a standard protein mix (50 µg) with 50 µl of MagReSyn® Trypsin in increasing concentrations of SDS. Lanes 1, 3, 5, 7, 9 & 11: Standard protein mix containing increasing concentrations of SDS, 0.01%, 0.02%, 0.1%, 0.25%, 0.5% & 1%; Lanes 2, 4, 6, 8, 10 and 12 are representative of digestion efficiencies of MagReSyn® Trypsin in the respective concentrations of SDS.
Digestion with MagReSyn® Trypsin provides good protein coverage for a range of proteins, including large and difficult to digest examples as Thyroglobulin and Myoglobin.
TERMS AND CONDITIONS
Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.