MagReSyn® NTA &
NTA Screening Kit
For rapid affinity purification of 6x-histidine-tagged proteins
MagReSyn® NTA microparticles consist of nitrilotriacetic acid (NTA) residues with a strong metal-chelating property. MagReSyn® NTA is pre-chelated with nickel ions for affinity purification of histidine-tagged proteins. The product has been engineered for exceptional specificity, delivering highly pure target proteins.
Since different types of His-tagged proteins may have varying affinities for a particular metal ion, we also offer a His-tagged protein purification screening kit (MagReSyn® NTA-KIT) containing MagReSyn® NTA chelated with 4 alternate divalent metal ions (Ni2+, Cu2+, Co2+, Zn2+) to identify the most suitable metal ion for optimal purification of your particular target protein. MagReSyn® NTA Copper, -Cobalt and -Zinc are currently available in 2 ml product formats.
Support: Proprietary polymer microspheres containing iron oxide (magnetite)
Binding capacity: > 1.0 mg.ml-1 (His-GFP)
Bead size: ~5-10 µm
Formulation: 25 mg.ml-1 suspension in 20% ethanol
MagReSyn® NTA Performance Superiority
(A) Improved purity of His-tagged target protein from crude E.coli cell lysate purified using MagReSyn® NTA. Lane 1: molecular weight markers, Lane 2: MagReSyn® NTA, Lane 3: Competitor 1, Lane 4: Competitor 2. All alternate products were used with supplier recommended buffers and protocols.
(B) Comparative assessment of MagReSyn® NTA (lane 2) against two leading alternate products (lanes 3 & 4). All samples were purified using the same binding/washing buffer (80 mM sodium phosphate pH 7,4, 40 mM imidazole, 1 M NaCI). 1 M NaCI). MagReSyn® microparticles showed a significant improvement in purity and recovery of the target protein.
(C) Specific enrichment of His-tagged target protein with crude E-coli cell lysate purified using MagReSyn® NTA. Lane 1: molecular weight markers, Lane 2: crude lysate, Lanes 3-6: 97% purity achieved in a single purification step. Results obtained for His-GFP isolated from crude E.coli lysate.
TERMS AND CONDITIONS
Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.
Citations and References
A Colorimetric Based Amplification System for Proteinases Including
MMP2 and ADAM8
– Marcia L. Moss et al.
ANALYTICAL BIOCHEMISTRY (2015)
Spectrophotometric activity microassay for pure and recombitant cytochrome P450-type nitric oxide reductase culture
– Seike Garny et al.
ANALYTICAL BIOCHEMISTRY 447 (2014)