MagReSyn® SAX

For rapid protein fractionation of proteins by ion exchange & concentration and clean-up of proteins from dilute solutions pre-MS analysis. SAX is used in the Mag-Net™ protocol for the enrichment of membrane vesicles from plasma and other biofluids.

MagReSyn^® SAX (strong anion exchange) is a magnetic polymeric microparticle support designed for capture, purification and recovery of biomolecules such as proteins/peptides, enzymes, antibodies by exploiting differences in net charge to reversibly adsorb oppositely-charged molecules. This product can be used for the capture of membrane bound vesicles using the Mag-Net™ protocol as described by the MacCoss Lab at University of Washington, department of Genome sciences has been benchmarked on the Astral™ Mass Spec by ThermoFisher (introduced at ASMS2023), and the Stellar™ Mass Spec (ASMS2024). Further information and resources related to this Mag-Net™ protocol can be found HERE.

MagReSyn^® SAX is ideal for fractionation of complex biological mixtures (e.g. serum, plasma, urine, CSF, cell lysates, culture supernatants etc.) prior to analytical methods such as 2-dimensional electrophoresis, HPLC or Mass Spectrometry. The microparticles have an exceptional capacity allowing for experimental miniaturization and elution in minimal volume.

Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Binding capacity: > 20 mg.ml^-1 (BSA) per ml
Bead size: ~5-20 µm
Formulation: 20 mg.ml^-1 suspension in 20% ethanol