MagReSyn® TiO2
TiO2 – functional microparticles for highly-specific phosphopeptide enrichment
Protein phosphorylation plays a pivotal role in most cellular processes, with 30% of the proteome transiently phosphorylated. It is therefore a widely studied post-translational modification. However, the low-abundant nature of phosphopeptides, the low stoichiometry of the modification, and the physico-chemical properties of phosphorylated peptides make proteome-wide characterization of phosphorylation a significant challenge to proteomics researchers.
Consequently, technologies than can specifically enrich phosphopeptides, and are compatible with mass spectrometric analyses, are highly desirable. MagReSyn® TiO2 microparticles allow highly specific, reproducible enrichment of phosphopeptides from complex biological samples such as protein digests. Titanium dioxide enrichment shows selective affinity for phosphoserine (pSer), phosphothreonine (pThr) and phosphotyrosine (pTyr) residues. MagReSyn® TiO2 microparticles have been engineered to achieve the ultimate specificity, outperforming competitor products, with excellent phosphopeptide recovery.
MagReSyn® TiO2 microparticles may be used alone or in combination with MagReSyn® ZrO2, MagReSyn® Zr-IMAC and/or MagReSyn® Ti-IMAC microparticles to enrich diverse types of phosphopeptides for comprehensive phosphoproteomics analyses. Products for phosphopeptide enrichment are validated for the intended application using our stringent QC procedures.
IMPORTANT NOTE: To ensure optimal enrichment using this product please ensure your sample is free of contaminating compounds by using sample preparation procedures such as PAC, and ensure sufficient mixing to ensure beads remain in suspension for good contact between your sample and the magnetic beads. Please read the instruction guide carefully before using the product, and ensure you centrifuge samples prior to LC MS analysis to remove possible insoluble contaminants.
Support: Proprietary polymer microparticles containing iron oxide (magnetite)
Bead size: ~5-10 µm
Formulation: 25 mg.ml-1 suspension in 20% ethanol
Product Resources
MagReSyn® TiO2 Performance Superiority
Comparative assessment of MagReSyn® TiO2 (green bars) vs a leading competitor (orange bars) for phosphopeptide enrichment.
32P labelled CHK1 tryptic digests were enriched for phosphopeptides and analyzed by LC/MS/MS.
MagReSyn® TiO2 showed exceptional specificity with up to 100% of identified peptides being phosphorylated, vs 78% for the competitor.
Phosphopeptide recovery was 78% using MagReSyn® TiO2 and 70% using alternate TiO2.
TERMS AND CONDITIONS
Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.
