MagReSyn® Streptavidin

High capacity for capture of biotinylated molecules

MagReSyn^® Streptavidin microparticles contain covalently coupled streptavidin. The porous nature of the polymer technology allows for exceptional streptavidin capacity, translating into increased capacity for the immobilization/capture of biotinylated biomolecules. The high streptavidin load allows the use of as little as 10 µl beads, improving experimental miniaturization for high-throughput automated analysis. Molecular applications of MagReSyn^® Streptavidin microparticles include, protein immobilization and purification; nucleic acid capture assays and preparation of single-stranded DNA templates for e.g. SELEX and pyrosequencing. The immobilization of biotinylated antibodies may subsequently be used in e.g. immunoassay.

Support: Proprietary polymer microparticles containing iron oxide (magnetite) with immobilised Streptavidin
Binding capacity (DNA): > 30 000 pmoles.ml^-1 (Biotinylated oligo, 25-mer)
Binding capacity (Protein): > 3 mg.ml^-1 (Biotinylated IgG)
Bead size: ~5-10 µm average
Formulation: 10 mg.ml^-1 suspension in sodium phosphate buffer

NEW: MagReSyn® Streptavidin MS (Mass Spectrometry)

Ultra-capacity for capture of biotinylated molecules from low cell counts, specifically for Mass Spectrometry workflows with reduced streptavidin peptides

MagReSyn® Streptavidin MS (Mass Spectrometry), is specifically designed to cater for applications in proteomics requiring on-bead enzymatic digestion of proteins using LysC and/or Trypsin, such as BioID, from low cell inputs. This is achieved through the chemical modification of lysine and arginine residues of Streptavidin as previously described by Rafiee et al (2020). The chemical modification of these residues has been optimized to retain a high affinity for Biotin, retaining over 80% of the capacity of our standard Streptavidin product, but with multiple-fold decreases in the release of streptavidin peptides resulting from on-bead digestion. For more information please read our app note in collaboration with ETH Zurich For the latest MS compatible conditions please consult our Method Library, and find relevant references by selecting “Streptavidin” from the product menu, and “on-bead” from the digestion menu.

Support: Proprietary polymer microparticles containing iron oxide (magnetite) with immobilised Streptavidin
Binding capacity (DNA): > 25 000 pmoles.ml^-1 (Biotinylated oligo, 25-mer)
Binding capacity (Protein): > 2.5 mg.ml^-1 (Biotinylated IgG)
Bead size: ~5-10 µm average
Formulation: 10 mg.ml^-1 suspension in sodium phosphate buffer

MagReSyn® Streptavidin MAX

Ultra-capacity for capture of biotinylated molecules

MagReSyn^® Streptavidin MAX microparticles have been specially engineered for custom applications where maximum binding of biotinylated molecules is required. This includes microplate applications where miniaturization and high-throughput automated analysis are required. This product primarily targeted for oligo/peptide isolation, and it is not recommended for protein isolation due to possible steric hindrance with the high streptavidin load. 

Binding capacity (DNA): > 120 000 pmoles.ml^-1 (Biotinylated oligo, 25-mer)
Bead size: ~5-10 µm
Formulation: 10 mg.ml^-1 suspension in sodium phosphate buffer

* Please note that MagReSyn^® Streptavidin MAX is a custom product with minimum 2 weeks lead time from date of order *