MagReSyn® Protein A Performance Superiority
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MagReSyn® Protein A
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High capacity Protein A for affinity purification of antibodies and immunoprecipitation
MagReSyn® Protein A MAX Ultra-capacity Protein A for the affinity purification of antibodies and immunoprecipitation MagReSyn® Protein A MAX microspheres have been specially engineered for custom applications where maximum antibody binding is desired. This includes microplate applications where miniaturization and high-throughput automated analysis are required. Binding capacity: > 4.8 mg.ml-1 (Rabbit IgG) Bead size: ~5-10 µm Formulation: 15 mg.ml-1 suspension in Tris buffer
* Please note that MagReSyn® Protein A MAX is a custom product and requires 2 weeks lead time from date of order *
Exceptional value o MagReSyn® Protein A binds over 4800 µg IgG per product compared to less than 3000 µg IgG for alternate products o MagReSyn® Protein A MAX binds over 9600 µg IgG per product
MagReSyn® Protein A microspheres provide superior binding capacity for IgG (over 160 µg.mg-1) compared to competitive suppliers. MagReSyn® Protein A MAX provides an unparalleled IgG binding capacity in excess of 320 µg.mg-1 for specialized applications. Green bars represent MagReSyn®, orange bars represent alternate suppliers
Vastly reduced Protein A leaching o Stable multipoint attachment of Protein A to the microspheres o Virtually no Protein A leaches during affinity capture and immunoglobulin elution
Protein A leaching was tested using a Protein A ELISA kit (GenScript) on comparative microsphere quantities required to purify 100 µg of rabbit IgG. Competitors’ products were evaluated using their recommended protocols. 
Specific enrichment of IgG from rabbit serum using MagReSyn® Protein A and alternate suppliers’ magnetic Protein A microspheres. The quantity of alternate suppliers’ microspheres was adjusted to equivalent binding capacity to compare the purity of the isolated IgG.  Lane 1: molecular weight markers, Lane 2: whole rabbit serum, Lane 3: MagReSyn® Protein A-purified IgG, Lane 4: Competitor 1, Lane 5: Competitor 2. MagReSyn® microspheres show significantly higher specificity for the target protein. IgG heavy and light chain fragments appear as two distinct bands on the gel (arrows).
Superior binding capacity for IgG o Unparalleled miniaturization of experimentation o Sample concentration from dilute solutions to a minimal volume
Exceptional specificity o Obtain IgG purity of >95% in a single chromatography step from whole serum samples o Eliminates the need for additional purification steps
Comparative assessment of IgG binding capacity of MagReSyn® Protein A (lane 8) against two leading competitors (lanes 6 & 7). All samples were purified using equal quantities of microspheres (1 mg each) and the suppliers’ recommended purification protocols and buffers. MagReSyn® microspheres show superior IgG capacity and purity (arrows). Lane 1: molecular weight markers, Lane 2: whole serum sample, Lanes 3-5: pooled unbound and wash fractions for the two competitors and MagReSyn® Protein A  respectively.
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MagReSyn® Protein A
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MagReSyn® Protein A microspheres contain covalently coupled Protein A for highly specific single-step purification of IgG to >95% purity from whole serum samples. Protein A is a ~56 kDa surface protein originally isolated from the cell wall of the bacterium Staphylococcus aureus and is widely used for highly specific purification of IgG antibodies and for immunoprecipitation experiments. Protein A has 5 immunoglobulin binding domains folded into a three-helix bundle and specifically binds the Fc region on the heavy chain of the IgG class of antibodies. More specifically, Protein A binds with high affinity to specific subclasses of human, rabbit, mouse, guinea pig, cat and pig IgG. It does not react with human IgG3, IgD or IgE, mouse IgM, IgA or IgE, or goat, koala and llama IgG. The high recombinant Protein A content of MagReSyn® ensures a high immunoglobulin capacity allowing for experimental miniaturization.  
Product Resources
Product Instruction Guide: Protein A Material Safety Data Sheet: Protein A Product Instruction Guide: Protein A MAX Material Safety Data Sheet: Protein A MAX
Support: Binding capacity: Bead size: Formulation:
Proprietary polymer microspheres containing iron oxide (magnetite) with immobilized Protein A > 2.4 mg.ml-1 (Rabbit IgG) ~5-10 µm 15 mg.ml-1 suspension in Tris buffer