MagReSyn® NTA Performance Superiority
(A) Improved purity of His-tagged target protein from crude E. coli cell lysate purified using MagReSyn® NTA. Lane 1: molecular weight markers, Lane 2: MagReSyn®  NTA, Lane 3: Competitor 1, Lane 4: Competitor 2. All competitor products were used with supplier recommended buffers and protocols. (B) Comparative assessment of MagReSyn® NTA (lane 2) against two leading competitors (lanes 3 & 4). All samples were purified using the same binding/washing buffer (80 mM sodium phosphate pH 7.4, 40 mM imidazole, 1 M NaCl). MagReSyn® microspheres show significant improvement in purity and recovery of the target protein.(C) Specific enrichment of His- tagged target protein from crude E. coli cell lysate purified using MagReSyn® NTA. Lane 1: molecular weight markers, Lane 2: crude lysate, Lanes 3-6:  MagReSyn® NTA- purified protein indicating batch-to-batch consistency, ensuring reproducible performance for protein purification. MagReSyn® microspheres show significant specificity for the target protein with >97% purity achieved in a single purification step. Results obtained for His-GFP isolated from crude E. coli lysate.
Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial purposes. Commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.
MagReSyn® NTA microparticles consist of nitrilotriacetic acid (NTA) residues with a strong metal- chelating property. MagReSyn® NTA is pre-chelated with nickel ions for affinity purification of histidine-tagged proteins. The product has been engineered for exceptional specificity, delivering highly pure target proteins. Since different types of His-tagged proteins may have varying affinities for a particular metal ion, we also offer a His-tagged protein purification screening kit (MagReSyn® NTA-KIT) containing MagReSyn® NTA chelated with 4 alternate divalent metal ions (Ni2+, Cu2+, Co2+, Zn2+) to identify the most suitable metal ion for optimal purification of your particular target protein. MagReSyn® NTA Copper, -Cobalt and -Zinc are currently available in 2 ml product formats. Support: Proprietary polymer microspheres containing iron oxide (magnetite) Binding capacity: > 1.0 (His-GFP) Bead size: ~5-10 µm Formulation: 25 suspension in 20% ethanol
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For rapid affinity purification of 6x-histidine-tagged proteins
MagReSyn® NTA &  NTA Screening Kit
1         2         3         4          1          2            3         4    1       2      3       4       5        6 A B C
Product Resources
Product Instruction Guide - MagReSyn® NTA