MagReSyn® Chymotrypsin Performance Superiority
Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial purposes. Commercial means any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.
MagReSyn® Chymotrypsin microspheres consist of covalently immobilized sequencing grade chymotrypsin (TLCK treated to prevent tryptic activity) for protein digestion prior to mass spectrometry analysis. The porous high capacity MagReSyn® microparticle technology allows for high volumetric enzyme activity allowing for rapid digestion. The magnetically immobilized chymotrypsin enables automated and miniaturized digestion workflows improving reproducibility. The enzyme cleaves at bulky hydrophobic residues thus providing complementary protein coverage as compared to magnetically immobilized trypsin digestion (see MagReSyn® Trypsin).   Support: Proprietary polymer microspheres containing iron oxide (magnetite) Digestion ratio: 20 µl (200 µg) microspheres per 50 µg protein sample Bead size: ~5-10 µm Formulation: 10 mg.ml-1 suspension in 50 mM acetic acid
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For rapid, automated chymotryptic digestion of proteins for mass spectrometry sample preparation
MagReSyn®  Chymotrypsin
Product Resources
Product Instruction Guide Material Safety Data Sheet O R ' R O H
Rapid protocols o High Chymotrypsin content ensures rapid digestion
Automation o Suitable for automated digestion on magnetic handling stations o Reduce sample preparation variability and improves reproducibility
Improved stability and compatibility o Multipoint covalent attachment of chymotrpysin o Low chymotrypsin leaching reduces sample contamination o Increased sequence coverage - complementary to trypsin cleavage
Please contact your local distributor or info@resynbio.com for a sample of this product for evaluation purposes
Digestion with MagReSyn® Chymotrypsin provides good protein coverage for a range of proteins, including large and difficult to digest examples such as Thyroglobulin and Myoglobin.
Application Note: Enhanced Sequence Coverage