MagReSyn® Chymotrypsin Performance Superiority
Products supplied by ReSyn Biosciences (Pty) Ltd are for research purposes only. ReSyn products are not to be used for diagnostic, therapeutic or commercial purposes. Commercial means
any use resulting in monetary gain, including, but not limited to, incorporation in a kit, repackaging and re-formulation. Please enquire about sub-licenses for commercial use.
MagReSyn® Chymotrypsin microspheres consist of covalently immobilized sequencing grade
chymotrypsin (TLCK treated to prevent tryptic activity) for protein digestion prior to mass
spectrometry analysis. The porous high capacity MagReSyn® microparticle technology allows for
high volumetric enzyme activity allowing for rapid digestion. The magnetically immobilized
chymotrypsin enables automated and miniaturized digestion workflows improving reproducibility.
The enzyme cleaves at bulky hydrophobic residues thus providing complementary protein
coverage as compared to magnetically immobilized trypsin digestion (see MagReSyn® Trypsin).
Support:
Proprietary polymer microspheres containing iron oxide (magnetite)
Digestion ratio:
20 µl (200 µg) microspheres per 50 µg protein sample
Bead size:
~5-10 µm
Formulation:
10 mg.ml-1 suspension in 50 mM acetic acid
© ReSyn Biosciences (Pty) Ltd 2012-2018
For rapid, automated chymotryptic digestion of proteins for mass
spectrometry sample preparation
MagReSyn®
Chymotrypsin
Product Resources
•
Rapid protocols
o
High Chymotrypsin content ensures rapid digestion
•
Automation
o
Suitable for automated digestion on magnetic handling stations
o
Reduce sample preparation variability and improves
reproducibility
•
Improved stability and compatibility
o
Multipoint covalent attachment of chymotrpysin
o
Low chymotrypsin leaching reduces sample contamination
o
Increased sequence coverage - complementary to trypsin cleavage
Please contact your local distributor or info@resynbio.com for a sample of this product for evaluation purposes
Digestion with MagReSyn® Chymotrypsin provides good protein coverage for a range
of proteins, including large and difficult to digest examples such as Thyroglobulin and
Myoglobin.
